LDLR Antibody (C7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80833

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Porcine, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Radioimmunoassay, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # C7

Format

Azide and BSA Free
View all LDLR Primary Antibodies »

Product Specifications

Immunogen

Partially purified bovine LDL receptor [Swiss-Prot# P01131].

Localization

Cell membrane/cell surface, endomembrane system, endosomes, lysosome, clathrin-coated pit, and Golgi apparatus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for LDLR Antibody (C7) - Azide and BSA Free

Western Blot: LDLR Antibody (C7)Azide and BSA Free [NBP2-80833]

Western Blot: LDLR Antibody (C7)Azide and BSA Free [NBP2-80833]

Western Blot: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]

Immunocytochemistry/Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]

Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]

Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]

Immunocytochemistry/Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue). Image from the standard format of this antibody.

Applications for LDLR Antibody (C7) - Azide and BSA Free

Application
Recommended Usage

Electron Microscopy

reported in scientific literature (PMID 11839845)

Flow Cytometry

reported in scientific literature (PMID 10906332)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 11839845)

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

In vitro assay

reported in scientific literature (PMID 9642270)

Western Blot

1:1000
Application Notes
In WB assay, specific bands can be seen around 160 kDa (mature form) and 120 kDa (precursor) molecular weight positions.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LDLR

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Gene Symbol

LDLR

Additional LDLR Products

Product Documents for LDLR Antibody (C7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for LDLR Antibody (C7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for LDLR Antibody (C7) - Azide and BSA Free

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Protocols

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FAQs

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Associated Pathways

A-beta Pathways: Uptake & Degradation
A-beta Pathways: Uptake & Degradation Thumbnail