LDLR Antibody (C7) - BSA Free

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) [NBP1-78159]

Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) [NBP1-78159]

Immunocytochemistry/Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - BSA Free [NBP1-78159] -

(A) Flowchart of the experiments used for testing the feasibility of the new system for enrichment of cells genome-edited at a single target locus. Four days after transfection with pCGsap1/LDLR, pgRNA#3, and pEGFP-N1, cells were treated with IB4SAP for a short period and cultured in normal medium for more than 10 days. The emerging colonies were propagated for molecular biological and cytochemical analyses; (B) cytochemical staining of clones LA-2 and -6 with AF594-IB4. Untransfected MPEFs were stained as positive control. Phase, photographs taken under light microscopy; AF594-IB4, photographs taken under UV illumination to detect AF594-IB4–derived red fluorescence. Bar = 30 μm; (C) electrophoretic pattern of polymerase chain reaction (PCR) products derived from the amplification of LA-1 to -7. Arrows indicate the size of PCR products for each gene (LDLR and GGTA1). M, 100 bp-ladder markers; (D) direct sequencing of PCR products from LA-2 (left panel) and LA-6 (right panel) using primers specific to LDLR or GGTA1 gene. Arrows above ideograms indicate the sites showing indels. In the bottom of each panel, sequencing results of inserts sub-cloned into TA cloning vector are shown. The numbers of clones examined are shown in parentheses. The translation initiation codon ATG is shown by boxes or in red. PAM is indicated by underlines. The deleted portion in the clones is shown by dotted lines; (E) immunocytochemistry of LA-6 and intact MPEFs (used as positive control) using anti-LDLR antibody. The parental MPEFs reactive to the second antibody (fluorescein-labeled anti-mouse IgG) alone are designated as negative control. All cells were counterstained with DAPI upon reaction with the second antibody. Bar = 30 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29207527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.