Leptin/OB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-59320

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Synthetic peptides corresponding to LEP(leptin) The peptide sequence was selected form the N terminal of LEP. Peptide sequence MHWGTLCGFLWLWPYLFYVQAVPIQKVQDDTKTLIKTIVTRINDISHTQS. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Leptin/OB Antibody - BSA Free

Western Blot: Leptin/OB Antibody [NBP1-59320]

Western Blot: Leptin/OB Antibody [NBP1-59320]

Western Blot: Leptin Antibody [NBP1-59320] - Titration: 0.2-1 ug/ml, Positive Control: Human Small Intestine.
Immunohistochemistry-Paraffin: Leptin/OB Antibody [NBP1-59320]

Immunohistochemistry-Paraffin: Leptin/OB Antibody [NBP1-59320]

Immunohistochemistry-Paraffin: Leptin Antibody [NBP1-59320] - Human placenta tissue at an antibody concentration of 5 ug/ml.

Applications for Leptin/OB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Leptin/OB

LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regula

Alternate Names

LEP, OB

Gene Symbol

LEP

OMIM

NP_000221 (Human)

UniProt

Additional Leptin/OB Products

Product Documents for Leptin/OB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Leptin/OB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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