Lgr5/GPR49 Antibody (CL13567) - BSA Free

Novus Biologicals | Catalog # NBP3-24570

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL13567

Format

BSA Free
View all Lgr5/GPR49 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from O75473, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Rat (87%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Lgr5/GPR49 Antibody (CL13567) - BSA Free

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human skeletal muscle shows moderate membranous positivity in myocytes.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Western Blot: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Western blot analysis in human cell line SH-SY5Y.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human ovarian cancer (dysgerminoma) shows strong membranous and cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human cerebral cortex shows moderate membranous positivity in a subset of neurons.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]

Analysis in human fallopian tube and tonsil tissues using NBP3-24570 antibody. Corresponding LGR5 RNA-seq data are presented for the same tissues.

Applications for Lgr5/GPR49 Antibody (CL13567) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lgr5/GPR49

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Gene Symbol

LGR5

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody (CL13567) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lgr5/GPR49 Antibody (CL13567) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Lgr5/GPR49 Antibody (CL13567) - BSA Free

There are currently no reviews for this product. Be the first to review Lgr5/GPR49 Antibody (CL13567) - BSA Free and earn rewards!

Have you used Lgr5/GPR49 Antibody (CL13567) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies