LMAN1 Antibody (OTI1A8)

Novus Biologicals | Catalog # NBP2-03381

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A8
View all LMAN1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 24891604). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMAN1 Antibody (OTI1A8)

Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunocytochemistry/Immunofluorescence: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381] - HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) wereimmunostained by anti-LMAN1 antibodyand then analyzed by flow cytometry.
Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381]

LMAN1-Antibody-OTI1A8-Western-Blot-NBP2-03381-img0012.jpg
Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Western Blot: LMAN1 Antibody (OTI1A8) [NBP2-03381] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Immunohistochemistry-Paraffin: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381]

Flow Cytometry: LMAN1 Antibody (OTI1A8) [NBP2-03381] - Analysis of Jurkat cells, using anti-LMAN1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
LMAN1 Antibody (OTI1A8)

LMAN1 Antibody (OTI1A8)[NBP2-03381]

LMAN1 Antibody (OTI1A8)[NBP2-03381]Simple Western� analysis of endogenous protein GALE from HepG2 lysates (0.2 mg/mL) using GALE Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for LMAN1 Antibody (OTI1A8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMAN1

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq].

Alternate Names

ERGIC-53, ERGIC53ER-Golgi intermediate compartment 53 kDa protein, F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53, FMFD1, Gp58, intracellular mannose specific lectin, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, lectin, mannose-binding, 1, MCFD1coagulation factor V-factor VIII combined deficiency, MR60, protein ERGIC-53

Entrez Gene IDs

3998 (Human)

Gene Symbol

LMAN1

Additional LMAN1 Products

Product Documents for LMAN1 Antibody (OTI1A8)

Certificate of Analysis

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Product Specific Notices for LMAN1 Antibody (OTI1A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LMAN1 Antibody (OTI1A8)

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Protocols

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