LMAN1 Antibody (OTI1A8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71152

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A8

Format

Azide and BSA Free
View all LMAN1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMAN1 Antibody (OTI1A8) - Azide and BSA Free

Western Blot: LMAN1 Antibody (OTI1A8)Azide and BSA Free [NBP2-71152]

Western Blot: LMAN1 Antibody (OTI1A8)Azide and BSA Free [NBP2-71152]

Western Blot: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staurosporine-induced cleavage of SMSr is sensitive to caspase-6 inhibitors. HeLa cells stably transduced with EV, SMSr-V5, and SMSrCR-V5 were treated with 1 ug/ml staurosporine for 6 h, lysed and subjected to immunoblot analysis using anti-V5, anti-PARP1
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunocytochemistry/Immunofluorescence: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Flow Cytometry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Flow Cytometry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) were stained by anti-LMAN1 antibody and then analyzed by flow cytometry.
Western Blot: LMAN1 Antibody (OTI1A8)Azide and BSA Free [NBP2-71152]

Western Blot: LMAN1 Antibody (OTI1A8)Azide and BSA Free [NBP2-71152]

Western Blot: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Western Blot: LMAN1 Antibody (OTI1A8)Azide and BSA Free [NBP2-71152]

Western Blot: LMAN1 Antibody (OTI1A8)Azide and BSA Free [NBP2-71152]

Western Blot: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.
Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Immunohistochemistry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Flow Cytometry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152]

Flow Cytometry: LMAN1 Antibody (OTI1A8) - Azide and BSA Free [NBP2-71152] - Analysis of Jurkat cells, using anti-LMAN1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for LMAN1 Antibody (OTI1A8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LMAN1

Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. LMAN1 is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. LMAN1 is involved in intracellular transport of many glycoproteins. LMAN1 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.

Alternate Names

ERGIC-53, ERGIC53ER-Golgi intermediate compartment 53 kDa protein, F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53, FMFD1, Gp58, intracellular mannose specific lectin, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, lectin, mannose-binding, 1, MCFD1coagulation factor V-factor VIII combined deficiency, MR60, protein ERGIC-53

Gene Symbol

LMAN1

Additional LMAN1 Products

Product Documents for LMAN1 Antibody (OTI1A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for LMAN1 Antibody (OTI1A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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