LRP-5 Antibody

Novus Biologicals | Catalog # NB100-1886

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LRP-5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ERVEKTTGDKRT corresponding to internal region according to NP_002326.2.

Reactivity Notes

This batch is not expected to cross-react with Mouse and Rat in Western blot.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LRP-5 Antibody

Western Blot: LRP-5 Antibody [NB100-1886]

Western Blot: LRP-5 Antibody [NB100-1886]

Western Blot: LRP-5 Antibody [NB100-1886] - (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: LRP-5 Antibody [NB100-1886]

Immunohistochemistry-Paraffin: LRP-5 Antibody [NB100-1886]

Immunohistochemistry-Paraffin: LRP-5 Antibody [NB100-1886] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for LRP-5 Antibody

Application
Recommended Usage

Immunohistochemistry

2-4 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml

Peptide ELISA

detection limit 1:16000

Western Blot

1-3 ug/ml
Application Notes
Western blot: Approx 180kDa band observed in lysates of cell line HepG2 (calculated MW of 179kDa according to NP_002326.2).
IHC: In paraffin embedded Human Liver shows weak cytoplasm staining in some hepatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRP-5

LRP5 is involved in the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. Defects in LRP5 are a cause of autosomal dominant and autosomal recessive familial exudative vitreoretinopathy (FEVR). Autosomal dominant FEVR is also referred to as exudative vitreoretinopathy 1 (EVR1); also known as Criswick-Schepens syndrome. FEVR is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. FEVR is reported to have a penetrance of 100%, but clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery.

Long Name

LDL Receptor-related Protein 5

Alternate Names

BMND1, EVR1, EVR4, HBM, LRP5, LRP7, OPPG, OPS, OPTA1, VBCH2

Entrez Gene IDs

4041 (Human)

Gene Symbol

LRP5

UniProt

O75197 (Human)

Additional LRP-5 Products

Product Documents for LRP-5 Antibody

Certificate of Analysis

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Product Specific Notices for LRP-5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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