Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Simple Western, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, ELISA, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B2E5

Format

BSA Free
View all Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]

Reactivity Notes

Human, mouse and monkey.

Localization

Nucleus

Marker

Nucleus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LSD1 Antibody (1B2E5) - BSA Free

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0007.jpg
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0006.jpg
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762]

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.
Simple Western: LSD1 Antibody (1B2E5) [NB100-1762]

Simple Western: LSD1 Antibody (1B2E5) [NB100-1762]

Simple Western: LSD1 Antibody (1B2E5) [NB100-1762] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.
Knockdown Validated: LSD1 Antibody (1B2E5) [NB100-1762]

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762]

LSD1-Antibody-1B2E5-Knockdown-Validated-NB100-1762-img0008.jpg

Applications for LSD1 Antibody (1B2E5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:500

Western Blot

1:500-1:2000
Application Notes

This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 120 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NB100-1762 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 1A/KDM1A/LSD1

The amine oxidase domain 2 AOF2 gene encodes a nuclear protein (LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serves as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.

Alternate Names

AOF2, BHC110, LSD1, Lysine (K)specific Demethylase 1A

Entrez Gene IDs

23028 (Human)

Gene Symbol

KDM1A

UniProt

O60341 (Human)

Additional Lysine (K)-specific Demethylase 1A/KDM1A/LSD1 Products

Product Documents for LSD1 Antibody (1B2E5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LSD1 Antibody (1B2E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for LSD1 Antibody (1B2E5) - BSA Free

Customer Reviews for LSD1 Antibody (1B2E5) - BSA Free (1)

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  • LSD1 Antibody (1B2E5) - BSA Free
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: glioblastoma cell lysates
    Species: Human
    Verified Customer | Posted 02/15/2026
    Control-treated or drug-treated glioblastoma cell lysate (10 ug) were subjected to western blot analysis. The dilution of antibody is 1/3000, overnight incubation, then detected using immobilon.
    LSD1 Antibody (1B2E5) - BSA Free NB100-1762

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Protocols

View specific protocols for LSD1 Antibody (1B2E5) - BSA Free (NB100-1762):

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for LSD1 Antibody (1B2E5) - BSA Free

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  • Q: I am interested in this product: LSD1 Antibody (NB100-1762). Could you tell me which epitope/domain this antibody recognizes? What is the immunogen used to generate this antibody? Is it a peptide? I just want to know the rough segment this antibody binds to. I am developing a sandwich ELISA for LSD1, and would like to have two LSD1 antibodies that recognizes different epitopes.

    A: The immunogen is a recombinant fragment of LSD1. Unfortunately the immunogen sequence is proprietary and I cannot disclose details about it. I can however tell you that it's found in the C-terminus of the protein. I hope this is helpful.

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail