Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32954

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Tagged Protein Expression

Species Reactivity

Validated:

Human, Feline

Cited:

Human

Predicted:

Bovine (96%), Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lysyl Oxidase Homolog 2/LOXL2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (83%). Feline reactivity reported from a verified customer review.

Localization

Secreted, extracellular space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian cancer. LOXL2 stained by LOXL2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: LOXL2 protein stained by LOXL2 antibody ) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human esophageal carcinoma.LOXL2 stained by LOXL2 antibody diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-Immunohistochemistry-Paraffin-NBP1-32954-img0043.jpg
ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (44.94-0.7 nM). Coated protein was probed with LOXL2 antibody (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Lysyl Oxidase Homolog 2/LOXL2 Antibody

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] -

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lysyl Oxidase Homolog 2/LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lysyl Oxidase Homolog 2/LOXL2 Antibody

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] -

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Lysyl Oxidase Homolog 2/LOXL2 antibody detects Lysyl Oxidase Homolog 2/LOXL2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Lysyl Oxidase Homolog 2/LOXL2 stained by Lysyl Oxidase Homolog 2/LOXL2 antibody (NBP1-32954) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Lysyl Oxidase Homolog 2/LOXL2 Antibody

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] -

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Western Blot: Rabbit Polyclonal Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954]

Whole cell lysates from SUM159 cells exposed to either 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. LOXL2 Antibody (NBP1-32954) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.
Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] -

Human LOXL2 protein, His tag (0.05 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free [NBP1-32954] -

Collagen organization and lysyl oxidase family of enzymes are differentially expressed andinterrelatedifferently in IPF. (A) Representative IHC images of lysyl oxidase enzymes in the lungs. Images are representative of lung tissues from non-IPF (n=10) and IPF (n=8) subjects (brown, protein of interest; blue, nucleus; pink, cytoplasm; scale bar: 200 um). (B) The ratio of LOX and LOXL1 percentage surface area obtained by image analysis of IHC-stained lung tissues of non-IPF (n=10; open circles) compared with IPF (n=8; filled diamonds) subjects; data were analysed with Student's two-tailed parametric unpaired t-test. (C) Correlation of the ratio of LOX and LOXL1 with F/B ratio obtained through image analysis of SHG microscopy images of collagen structure in non-IPF (n=7) and IPF (n=8) subjects (P<0.0001; R2=0.7527; average of three or four samples per subject). (D) 3D scatter plot of collagen organization (intensity F/B, i.e. SHG intensity forward/backward signal ratio), LOXL1 and LOXL2 expression (open circles, non-IPF, n=7; filled circles, IPF, n=8). (E) Scree plot showing the number of relationship groups needed to explain the associations within measured variables in non-IPF and IPF tissues (eigenvalue threshold≥1). (F,G) Weighted average values of the variables contributing to each relationship group; comparing non-IPF with IPF subjects (average of three or four samples per subject); data were analysed with Student's two-tailed parametric unpaired t-test. Data are presented as means+/-s.d. Data were obtained from one experimental replicate per subject. *P<0.05, **P<0.01, ***P<0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29125826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 3 reviews rated 3.7 using NBP1-32954 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase Homolog 2/LOXL2

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Alternate Names

LOL2, LOXL2

Entrez Gene IDs

4017 (Human); 94352 (Mouse)

Gene Symbol

LOXL2

UniProt

Additional Lysyl Oxidase Homolog 2/LOXL2 Products

Product Documents for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Customer Reviews for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free (3)

3.7 out of 5
3 Customer Ratings
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Customer Images


Showing  1 - 3 of 3 reviews Showing All
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  • Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: SUM-159PT human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/17/2025
    Western Blot: whole cell lysates from SUM159 cells exposed to either 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. LOXL2 Antibody (NBP1-32954) was used for primary antibody: 1:1000, 4℃, overnight.
    Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free NBP1-32954
  • Lysyl Oxidase Homolog 2/LOXL2 Antibody
    Name: Junhua Yang
    Application: Simple Western
    Sample Tested: Huh7 whole cell lysate
    Species: Human
    Verified Customer | Posted 01/25/2023
    https://www.novusbio.com/sites/novusbio.com/files/reviews/Picture1_0.jpg
    Cell line: Huh7 (HCC) Cell lysate buffer:50mM Tris/150mM Nacl/0.5% NP40/PI LOXL2 antibody dilution tried 1:100 and 1:200, the 1:200 is right dilution Sample dilution:0.2 mg/ml and 1mg/ml. 1mg/ml is better
    Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free NBP1-32954
  • Lysyl Oxidase Homolog 2/LOXL2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: cat
    Verified Customer | Posted 01/14/2019
    Feline cardiac fibroblasts, with and without LOXL2 antibody.
    Feline cardiac fibroblasts were blocked in 100µl of 10% Goat serum for 30 min and 0.85µl of LOXL2 antibody was added in each well in the 24-well plate. The cells were incubated with the primary antibody at 4C overnight, then were washed 3 times with PBST (5 min each). Goat anti rabbit IgG conjugated with Cy2 were used as secondary. DAPI was also added in for staining of the nuclei.
    Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free NBP1-32954

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Protocols

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FAQs for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

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  • Q: What is the appropriate positive control for NBP1-32954 in immunohistochemistry on paraffin embedded sections?

    A: According to The Human Protein Atlas Lysyl oxidase homolog 2 protein is expressed in most tissues. You can use any of the tissues with good (at least medium) protein expression levels as your positive control for IHC. Please see the graph and examples of IHC tissue stainings here: https://www.proteinatlas.org/ENSG00000134013-LOXL2/tissue. For example, I see the references for this product successfully stained human lung tissue, ovarian and breast carcinomas. If you wish to purchase a tissue slide to be used as a control, we have some in our catalog. You can find them here and here

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