Max Antibody

Novus Biologicals | Catalog # NB100-793

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-EEPQSRKKLRMEAS corresponding to C-Terminus according to NP_002373.3, NP_660087.1.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24676840).

Specificity

This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat Max Antibody (NB100-793) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Max Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Max Antibody

Western Blot: Max Antibody [NB100-793]

Western Blot: Max Antibody [NB100-793]

Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Max Antibody [NB100-793]

Immunohistochemistry-Paraffin: Max Antibody [NB100-793]

Immunohistochemistry-Paraffin: Max Antibody [NB100-793] - Staining of Prostate. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence: Max Antibody [NB100-793]

Immunofluorescence: Max Antibody [NB100-793]

Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence: Max Antibody [NB100-793]

Immunofluorescence: Max Antibody [NB100-793]

Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for Max Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx 22 kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3 kDa according to NP_002373.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Max

A basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD - regulating gene expression.

Long Name

Myc associated factor X

Alternate Names

BHLHD4, bHLHd4MGC11225, bHLHd5, bHLHd6, bHLHd7, bHLHd8, Class D basic helix-loop-helix protein 4, helix-loop-helix zipper protein, MAX protein, MGC10775, MGC18164, MGC34679, MGC36767, MYC associated factor X, Myc-associated factor X, orf1, protein max

Entrez Gene IDs

4149 (Human); 17187 (Mouse)

Gene Symbol

MAX

UniProt

P61244

Additional Max Products

Product Documents for Max Antibody

Certificate of Analysis

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Product Specific Notices for Max Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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