MBP Antibody (2H9) - BSA Free

Novus Biologicals | Catalog # NBP2-22121

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry, In vivo assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H9

Format

BSA Free
View all MBP Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]

Reactivity Notes

Mouse reactivity reported by customer review.

Marker

Oligodendrocyte Marker, Myelin Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MBP Antibody (2H9) - BSA Free

Western Blot: MBP Antibody (2H9)BSA Free [NBP2-22121]

Western Blot: MBP Antibody (2H9)BSA Free [NBP2-22121]

Western Blot: MBP Antibody (2H9) [NBP2-22121] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/mL and detected using an anti-mouse HRP labeled secondary antibody and chemiluminescent substrate.
Immunocytochemistry/ Immunofluorescence: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunocytochemistry/ Immunofluorescence: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunocytochemistry/Immunofluorescence: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Frozen: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunohistochemistry-Frozen: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunohistochemistry-Frozen: MBP Antibody (2H9) [NBP2-22121] - Mouse brain cryosections were stained with anti-MBP (1:400) and anti-mouse Alexa Fluor 555 IgG. Staining specific, hardly any background. Magnification 1:20. Image from verified customer review.
Flow Cytometry: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Flow Cytometry: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Flow Cytometry: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunohistochemistry-Paraffin: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunohistochemistry-Paraffin: MBP Antibody (2H9) - BSA Free [NBP2-22121]

Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining.
ELISA: MBP Antibody (2H9) - BSA Free [NBP2-22121]

ELISA: MBP Antibody (2H9) - BSA Free [NBP2-22121]

ELISA: Myelin Basic Protein Antibody (2H9) [NBP2-22121] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Applications for MBP Antibody (2H9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Frozen

reported by customer review

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-22121 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human)

Gene Symbol

MBP

UniProt

P02686

Additional MBP Products

Product Documents for MBP Antibody (2H9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MBP Antibody (2H9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

Citations for MBP Antibody (2H9) - BSA Free

Customer Reviews for MBP Antibody (2H9) - BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
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3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

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  • MBP Antibody (2H9)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 05/09/2019
    Mouse brain cryosections were stained with anti-MBP (1:400) and anti-mouse Alexa Fluor 555 IgG. Staining specific, hardly any background. Magnification 1:20.
    MBP Antibody (2H9) - BSA Free NBP2-22121
  • MBP Antibody (2H9)
    Name: Jan Martinek
    Application: Immunofluorescence Frozen Acetone fixed
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 12/07/2018
    Mouse brain section stained for MBP (red) and counterstained with DAPI (blue)
    MBP Antibody (2H9) - BSA Free NBP2-22121

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Protocols

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FAQs for MBP Antibody (2H9) - BSA Free

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  • Q: Has this antibody been tested on mouse and rat tissues/cells to show exclusive staining to human cells?

    A: For our product NBP2-22121, we have only tested this product in human samples thus far. We have not yet tested for cross reactivity with the mouse or rat proteins. The immunogen for this product shares 70% homology to the mouse sequence and 80% to the rat.

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