MBP Antibody (2H9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80844

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H9

Format

Azide and BSA Free
View all MBP Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MBP Antibody (2H9) - Azide and BSA Free

Western Blot: MBP Antibody (2H9)Azide and BSA Free [NBP2-80844]

Western Blot: MBP Antibody (2H9)Azide and BSA Free [NBP2-80844]

Western Blot: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/mL and detected using an anti-mouse HRP labeled secondary antibody and chemiluminescent substrate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunocytochemistry/ Immunofluorescence: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunocytochemistry/Immunofluorescence: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunohistochemistry-Paraffin: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunohistochemistry-Paraffin: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining. Image from the standard format of this antibody.
Flow Cytometry: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Flow Cytometry: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Flow Cytometry: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red). Image from the standard format of this antibody.
Immunohistochemistry-Frozen: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunohistochemistry-Frozen: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunohistochemistry-Frozen: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Mouse brain cryosections were stained with anti-MBP (1:400) and anti-mouse Alexa Fluor 555 IgG. Staining specific, hardly any background. Magnification 1:20. Image from the standard format of this antibody. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunohistochemistry-Paraffin: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

Immunohistochemistry-Paraffin: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining. Image from the standard format of this antibody.
ELISA: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

ELISA: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844]

ELISA: MBP Antibody (2H9) - Azide and BSA Free [NBP2-80844] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng). Image from the standard format of this antibody.

Applications for MBP Antibody (2H9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Frozen

reported by customer review

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Gene Symbol

MBP

Additional MBP Products

Product Documents for MBP Antibody (2H9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MBP Antibody (2H9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

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Protocols

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FAQs

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