MED12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86669

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MED12 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MED12 Antibody [NBP1-86669]

Immunocytochemistry/ Immunofluorescence: MED12 Antibody [NBP1-86669]

Immunocytochemistry/Immunofluorescence: MED12 Antibody [NBP1-86669] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining in human placenta and pancreas tissues using anti-MED12 antibody. Corresponding MED12 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human cerebral cortex, colon, spleen and testis using Anti-MED12 antibody NBP1-86669 (A) shows similar protein distribution across tissues to independent antibody NBP1-86670 (B).
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human colon.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human spleen.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669]

Immunohistochemistry-Paraffin: MED12 Antibody [NBP1-86669] - Staining of human testis.
MED12 Antibody - BSA Free Western Blot: MED12 Antibody - BSA Free [NBP1-86669]

Western Blot: MED12 Antibody - BSA Free [NBP1-86669]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MED12 antibody. Remaining relative intensity is presented.
MED12 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MED12 Antibody - BSA Free [NBP1-86669]

Chromatin Immunoprecipitation-exo-Seq: MED12 Antibody - BSA Free [NBP1-86669]

ChIP-Exo-Seq composite graph for Anti-MED12 (NBP1-86669) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for MED12 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED12

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. (provided by RefSeq)

Long Name

Mediator of RNA Polymerase II Transcription Subunit 12

Alternate Names

ARC240, CAGH45, FGS1, HOPA, OKS, TNRC11, TRAP230

Gene Symbol

MED12

Additional MED12 Products

Product Documents for MED12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MED12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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