MEF2C Antibody (CL0368)

Novus Biologicals | Catalog # NBP3-52062

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0368
View all MEF2C Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q06413, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MEF2C Antibody (CL0368)

MEF2C Antibody (CL0368) Western Blot: MEF2C Antibody (CL0368) [NBP3-52062]

Western Blot: MEF2C Antibody (CL0368) [NBP3-52062]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)�
Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419349)
MEF2C Antibody (CL0368) Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.
MEF2C Antibody (CL0368) Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
MEF2C Antibody (CL0368) Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of human tonsil shows strong nuclear immunoreactivity in a subset of lymphoid cells.
MEF2C Antibody (CL0368) Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of human skeletal muscle shows moderate nuclear positivity in muscle fibers.
MEF2C Antibody (CL0368) Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of human duodenum shows strong nuclear immunoreactivity in a subset of lymphoid cells.
MEF2C Antibody (CL0368) Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0368) [NBP3-52062]

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of RH-30 cells showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
MEF2C Antibody (CL0368) Western Blot: MEF2C Antibody (CL0368) [NBP3-52062]

Western Blot: MEF2C Antibody (CL0368) [NBP3-52062]

Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
MEF2C Antibody (CL0368) Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP3-52062]

Staining of human pancreas shows absence of staining (negative control).

Applications for MEF2C Antibody (CL0368)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture. Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

Additional MEF2C Products

Product Documents for MEF2C Antibody (CL0368)

Certificate of Analysis

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Product Specific Notices for MEF2C Antibody (CL0368)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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