MKK7/MEK7 Antibody (2G5) - Azide and BSA Free

Name: MKK7/MEK7 Antibody (2G5) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005609-M04
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005609-M04

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 2G5

Format

Azide and BSA Free

View all MKK7 Primary Antibodies »

Product Specifications

Immunogen

MAP2K7 (NP_660186, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQHPTPPARPRHMLGLPSTLFTPRSMESIEIDQKLQEI

Localization

Cytoplasmic and Nuclear

Specificity

MAP2K7 - mitogen-activated protein kinase kinase 7

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MKK7/MEK7 Antibody (2G5) - Azide and BSA Free

Western Blot: MKK7/MEK7 Antibody (2G5) [H00005609-M04]

Western Blot: MKK7/MEK7 Antibody (2G5) [H00005609-M04] - Analysis of MAP2K7 expression in transfected 293T cell line by MAP2K7 monoclonal antibody (M04), clone 2G5. Lane 1: MAP2K7 transfected lysatE (47.485 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: MKK7/MEK7 Antibody (2G5) [H00005609-M04]

Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (2G5) [H00005609-M04] - Analysis of monoclonal antibody to MAP2K7 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: MKK7/MEK7 Antibody (2G5) [H00005609-M04]

Immunohistochemistry-Paraffin: MKK7/MEK7 Antibody (2G5) [H00005609-M04] - Analysis of monoclonal antibody to MAP2K7 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 1.2 ug/ml

Proximity Ligation Assay: MKK7/MEK7 Antibody (2G5) [H00005609-M04]

Proximity Ligation Assay: MKK7/MEK7 Antibody (2G5) [H00005609-M04] - Analysis of protein-protein interactions between MAPK8 and MAP2K7. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-MAP2K7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interacti

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Applications for MKK7/MEK7 Antibody (2G5) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500

Application Notes

Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKK7

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.

Long Name

Mitogen-activated Protein Kinase Kinase 7

Alternate Names

JNKK2, MAP2K7, MKE7, PRKMK7

Entrez Gene IDs

5609 (Human)

Gene Symbol

MAP2K7

OMIM

603014 (Human)

UniProt

NP_660186 (Human)

Additional MKK7 Products

Product Documents for MKK7/MEK7 Antibody (2G5) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for MKK7/MEK7 Antibody (2G5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.