MKK7/MEK7 Antibody (4E5)

Name: MKK7/MEK7 Antibody (4E5)
Brand: Novus Biologicals
SKU: NBP2-37542
Price: 159 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37542

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4E5

View all MKK7 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MKK7/MEK7 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKK7/MEK7 Antibody (4E5)

Western Blot: MKK7/MEK7 Antibody (4E5)BSA Free [NBP2-37542]

Western Blot: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)

Immunocytochemistry/ Immunofluorescence: MKK7/MEK7 Antibody (4E5) - BSA Free [NBP2-37542]

Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunofluorescence analysis of Hela cells using MAP2K7 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: MKK7/MEK7 Antibody (4E5) - BSA Free [NBP2-37542]

Immunohistochemistry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K7 mouse mAb with DAB staining.

Flow Cytometry: MKK7/MEK7 Antibody (4E5) - BSA Free [NBP2-37542]

Flow Cytometry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Flow cytometric analysis of Hela cells using MAP2K7 mouse mAb (green) and negative control (red).

Immunohistochemistry: MKK7/MEK7 Antibody (4E5) - BSA Free [NBP2-37542]

Immunohistochemistry: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MAP2K7 mouse mAb with DAB staining.

ELISA: MKK7/MEK7 Antibody (4E5) - BSA Free [NBP2-37542]

ELISA: MKK7/MEK7 Antibody (4E5) [NBP2-37542] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

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Applications for MKK7/MEK7 Antibody (4E5)

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK7

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Long Name

Mitogen-activated Protein Kinase Kinase 7

Alternate Names

JNKK2, MAP2K7, MKE7, PRKMK7

Gene Symbol

MAP2K7

Additional MKK7 Products

Product Documents for MKK7/MEK7 Antibody (4E5)

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Certificate of Analysis

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Product Specific Notices for MKK7/MEK7 Antibody (4E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.