MNK1 Antibody (2F12) - Azide and BSA Free

Novus Biologicals | Catalog # H00008569-M14

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F12

Format

Azide and BSA Free
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Product Specifications

Immunogen

MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MAP kinase interacting serine/threonine kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MNK1 Antibody (2F12) - Azide and BSA Free

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

MNK1-Antibody-2F12-Western-Blot-H00008569-M14-img0010.jpg
Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14]

Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14]

Immunocytochemistry/Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14]

Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14]

Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - detection against Immunogen (77.26 KDa).
Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14]

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of MKNK1 expression in Raw 264.7. (Isoform: 51.342, 47.402, 39.103 kD)
MNK1 Antibody (2F12)

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] -

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - p38 alpha -mediated phosphorylation pathways.a. Old wild type & p38 alpha knock out livers were Western blotted for phosphorylated MKK3/6 & MKK4, for phosphorylated p38 & total levels of p38 alpha. Densitometric quantification of p-MKK3/6 (S189/207)/ alpha -tubulin, p-MKK4 (S257/T261)/a tubulin were done. b. Old wild type & p38 alpha knock out livers were Western blotted for MNK1 & phosphorylated MNK1 on Thr197/202, for MK2 & phosphorylated MK2 on Thr334 & Thr222, for AKT & phosphorylated AKT on Ser473, for GSK3 beta & phosphorylated GSK3 beta on serine 9 & for HSP27 & phosphorylated HSP27 on Ser 82. alpha -tubulin was used as a loading control. Densitometric quantification of p-MNK-1(T197/202)/MNK1, p-MK2 (T334)/MK2, p-MK2 (T222) /MK2, p-AKT(S473)/AKT, p-GSK3 beta (S9)/GSK3 beta & p-HSP27(S82)/HSP27 were determined. Data are shown as mean ± SD. **P < 0.01 WT versus KO. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0171738), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MNK1 Antibody (2F12) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MNK1

MNK1 may play a role in the response to environmental stress and cytokines. It appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Long Name

MAP kinase-interacting serine/threonine-protein kinase 1

Alternate Names

MKNK1

Entrez Gene IDs

8569 (Human)

Gene Symbol

MKNK1

OMIM

606724 (Human)

Additional MNK1 Products

Product Documents for MNK1 Antibody (2F12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MNK1 Antibody (2F12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MNK1 Antibody (2F12) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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