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Mouse 5'‑Nucleotidase/CD73 APC‑conjugated Antibody

  • Specificity
    Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 496406
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
    Trp29-Lys549
    Accession # Q61503
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of 5'‑Nucleotidase/CD73 in CD4+ Mouse Splenocytes by Flow Cytometry. CD4+ mouse splenocytes were stained with Rat Anti-Mouse 5'-Nucleotidase/CD73 APC-conjugated Monoclonal Antibody (Catalog # FAB4488A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

  • References:
    1. Resta, R. et al. (1993) Gene 133:171.
    2. Resta, R. et al. (1998) Immunol. Rev. 161:95.
    3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
  • Entrez Gene IDs:
    23959 (Human); 4907 (Human); 58813 (Rat);
  • Alternate Names:
    5-NT; CD73; E5NT; eNT; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5'-nucleotidase, ecto (CD73); CD73; CD73 antigen; E5NT; EC 3.1.3.5; Ecto-5'-nucleotidase; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NTE; Purine 5-Prime-Nucleotidase
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