Mouse ACE/CD143 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1513
Product Details
Citations (1)
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Mouse ACE/CD143 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ACE/CD143 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant mouse ACE-2 and recombinant human ACE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ACE/CD143
Leu35-Gln1264
Accession # P09470
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ACE/CD143 Somatic Form (Catalog # 1513-ZN)
Flow Cytometry
2.5 µg/106 cells
Mouse lung single‑cell suspension
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse kidney

Mouse ACE/CD143 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse ACE/CD143 Antibody (Catalog # MAB15132)

Standard: Recombinant Mouse ACE Protein, CF (Catalog # 1513-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE/CD143

ACE (also known as peptidyl-dipetidase A) is a zinc metallopeptidase important for blood pressure control and water and salt metabolism (1). It cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II and inactivates bradykinin by the sequential removal of two C-terminal dipeptides. In addition to the two physiological substrates, ACE cleaves C-terminal dipeptides from various oligopeptides with a free C-terminus. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid beta -peptide (A beta ), retards A beta aggregation, deposition, fibril formation, and inhibits cytotoxicity (2).

ACE is a type I membrane protein and exists in two isoforms (1). Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar catalytic domains called N- and C-domains. Germinal ACE, found exclusively in the testes, comprises a single catalytic domain identical to the C-domain of somatic ACE except for an N-terminal 67 residue germinal ACE-specific sequence. Physiological functions of the two tissue-specific isozymes are not interchangeable (3). For example, sperm-specific expression of the germinal ACE, not the somatic ACE, in ACE knockout male mice restored fertility.

Soluble ACE is present in many biological fluids, such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid (1). The soluble ACE is derived from the membrane forms by actions of secretases or sheddases. The identities of the secretases have not been revealed, although they belong to the family of zinc metallopeptidases (4, 5).

References
  1. Corvol, P. et al. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.) p. 332, Academic Press, San Diego.
  2. Hu, J. et al. (2001) J. Biol. Chem. 276:47863.
  3. Kessler, S.P. et al. (2000) J. Biol. Chem. 275:26259.
  4. Eyries, M. et al. (2001) J. Biol. Chem. 276:5525.
  5. Alfalah, M. et al. (2001) J. Biol. Chem. 276:21105.
Long Name
Angiotensin I Converting Enzyme
Entrez Gene IDs
1636 (Human); 11421 (Mouse)
Alternate Names
ACE; ACE1angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; carboxycathepsin; CD143 antigen; CD143; DCP; DCP1; DCP1angiotensin-converting enzyme; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; EC 3.2.1.-; EC 3.4.15.1; Kininase II; MGC26566; MVCD3; peptidase P; testicular ECA

Product Datasheets

Citation for Mouse ACE/CD143 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Reduced severity of a mouse colitis model with angiotensin converting enzyme inhibition.
    Authors: Spencer AU, Yang H, Haxhija EQ, Wildhaber BE, Greenson JK, Teitelbaum DH
    Dig. Dis. Sci., 2007;52(4):1060-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

  1. Is ACE-1 the same as ACE?

    • Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

View all Antibody FAQs

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