Mouse Adiponectin/Acrp30 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects full-length mouse Adiponectin (aa 18‑247) in direct ELISAs and Western blots. Shows no cross-reactivity with recombinant human Adiponectin. Does not detect the C-terminal globular domain of mouse Adiponectin (aa 111‑247).
  • Source
    Monoclonal Rat IgG2A Clone # 173205
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin
    Glu18-Asn247
    Accession # Q60994
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of mouse adipose tissue. PVDF membrane was probed with 2 µg/mL of Mouse Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB1119) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Adiponectin/Acrp30 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1 - 3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4 - 7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8 - 10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11 - 13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

  • References:
    1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
    2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
    3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
    4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
    5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
    6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
    7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
    8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
    9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
    10. Waki, H. et al. (2005) Endocrinology 146:790.
    11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
    12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
    13. Wang, Y. et al. (2008) Biochem. J. 409:623.
    14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
    15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
  • Entrez Gene IDs:
    9370 (Human); 11450 (Mouse); 246253 (Rat)
  • Alternate Names:
    ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Adiponectin reduces plasma triglyceride by increasing VLDL triglyceride catabolism.
    Authors: Qiao L, Zou C, van der Westhuyzen DR, Shao J
    Diabetes, 2008;57(7):1824-33.
    Species: Mouse
    Sample Type: Serum
    Application: WB
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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