Adiponectin (Acrp30) is secreted primarily by adipocytes and induces multiple paracrine and endocrine effects on metabolism and inflammation. Adiponectin promotes insulin sensitivity, decreased serum glucose and triglyceride levels, and increased serum glucagon levels. It promotes fatty acid uptake and oxidation, glucose uptake, and lactate production in skeletal muscle. Adiponectin promotes the development of M2 macrophages and promotes the C1qRp mediated clearance of apoptotic cell debris. Decreased plasma HMW Adiponectin levels are associated with upper body obesity, insulin resistance, reduced fatty acid oxidation, dyslipidemia, coronary artery disease, and atherogenesis. Adiponectin forms homotrimers, hexamers, and high molecular weight (HMW) complexes and signals through the receptors AdipoR1 and AdipoR2.
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Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
R&D Systems | Catalog # MRP300
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.007 ng/mL
Assay Range
0.16-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
The Quantikine Mouse Adiponectin Immunoassay is a 4.5 hour solid phase ELISA designed to measure full-length mouse Adiponectin levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains recombinant mouse Adiponectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse Adiponectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant full-length mouse Adiponectin. This assay only recognizes full-length mouse Adiponectin. It does not recognize mouse gAdiponectin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 23 | 23 | 23 |
| Mean (ng/mL) | 0.33 | 1.23 | 3.83 | 0.35 | 1.22 | 4.02 |
| Standard Deviation | 0.022 | 0.072 | 0.221 | 0.021 | 0.061 | 0.258 |
| CV% | 6.7 | 5.9 | 5.8 | 6.0 | 5.0 | 6.4 |
Recovery for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
The recovery of mouse Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 103 | 82-115 |
| EDTA Plasma (n=4) | 92 | 81-106 |
| Heparin Plasma (n=4) | 93 | 77-110 |
| Serum (n=6) | 98 | 85-114 |
Linearity
To assess the linearity of the assay, samples containing mouse Adiponectin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
Mouse Adiponectin/Acrp30 ELISA Standard Curve
Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
Changes in steroid hormone levels and adipokine levels in peripheral blood. (a) Testosterone. (b) Estradiol. (c) Corticosterone. (d) DHEA. (e) Leptin. (f) Adiponectin. For all groups, n = 8. Values are expressed as mean ± standard error. *P < 0.05, significantly different from the 3moR group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-017-12358-8), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
Additional Adiponectin/Acrp30 Products
Product Documents for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit
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6 Customer Ratings
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Sample Tested: mouse plasma/EDTA and EDTA PlasmaVerified Customer | Posted 06/23/2023
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Sample Tested: SerumVerified Customer | Posted 05/18/2023
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Sample Tested: SerumVerified Customer | Posted 03/04/2022
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Sample Tested: EDTA PlasmaVerified Customer | Posted 08/27/2021
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Sample Tested: Pancreatic cancer cellsVerified Customer | Posted 04/26/2018
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Sample Tested: 4T1 mouse breast cancer cell lineVerified Customer | Posted 06/05/2017
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Protocols
View specific protocols for Mouse Adiponectin/Acrp30 Quantikine ELISA Kit (MRP300):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
