Mouse Adiponectin/Acrp30 Quantikine ELISA Kit

  (42 citations)
(2 Reviews)
    
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Product Details
Assay Procedure
Citations (42)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.007 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant full-length mouse Adiponectin. This assay only recognizes full-length mouse Adiponectin. It does not recognize mouse gAdiponectin.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Adiponectin Immunoassay is a 4.5 hour solid phase ELISA designed to measure full-length mouse Adiponectin levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse Adiponectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse Adiponectin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 23 23 23
Mean 0.33 1.23 3.83 0.35 1.22 4.02
Standard Deviation 0.022 0.072 0.221 0.021 0.061 0.258
CV% 6.7 5.9 5.8 6 5 6.4

Recovery

The recovery of mouse Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 103 82-115
EDTA Plasma (n=4) 92 81-106
Heparin Plasma (n=4) 93 77-110
Serum (n=6) 98 85-114
Linearity
To assess the linearity of the assay, samples containing mouse Adiponectin in each matrix were diluted with Calibrator Diluent and then assayed.
 Adiponectin/Acrp30 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

  • Entrez Gene IDs:
    9370 (Human); 11450 (Mouse); 246253 (Rat)
  • Alternate Names:
    ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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Species
Sample Type
  1. Epigenetic modulation of Fgf21 in the perinatal mouse liver ameliorates diet-induced obesity in adulthood
    Authors: X Yuan, K Tsujimoto, K Hashimoto, K Kawahori, N Hanzawa, M Hamaguchi, T Seki, M Nawa, T Ehara, Y Kitamura, I Hatada, M Konishi, N Itoh, Y Nakagawa, H Shimano, T Takai-Igar, Y Kamei, Y Ogawa
    Nat Commun, 2018;9(1):636.
    Species: Mouse
    Sample Type: Serum
  2. Sterol regulatory element-binding protein-1c orchestrates metabolic remodeling of white adipose tissue by caloric restriction
    Authors: N Fujii, T Narita, N Okita, M Kobayashi, Y Furuta, Y Chujo, M Sakai, A Yamada, K Takeda, T Konishi, Y Sudo, I Shimokawa, Y Higami
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Plasma
  3. Adipose Tissue Deficiency of Hormone-Sensitive Lipase Causes Fatty Liver in Mice
    Authors: B Xia, GH Cai, H Yang, SP Wang, GA Mitchell, JW Wu
    PLoS Genet., 2017;13(12):e1007110.
    Species: Mouse
    Sample Type: Plasma
  4. ASK1 (MAP3K5) is transcriptionally upregulated by E2F1 in adipose tissue in obesity, molecularly defining a human dys-metabolic obese phenotype
    Authors: Y Haim, M Blüher, D Konrad, N Goldstein, N Klöting, I Harman-Boe, B Kirshtein, D Ginsberg, T Tarnovscki, Y Gepner, I Shai, A Rudich
    Mol Metab, 2017;6(7):725-736.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  5. Chronic Psychological Stress Accelerates Vascular Senescence and Impairs Ischemia-Induced Neovascularization: The Role of Dipeptidyl Peptidase-4/Glucagon-Like Peptide-1-Adiponectin Axis
    Authors: L Piao, G Zhao, E Zhu, A Inoue, R Shibata, Y Lei, L Hu, C Yu, G Yang, H Wu, W Xu, K Okumura, N Ouchi, T Murohara, M Kuzuya, XW Cheng
    J Am Heart Assoc, 2017;6(10):.
    Species: Mouse
    Sample Type: Plasma
  6. Exercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis
    Authors: S Komine, K Akiyama, E Warabi, S Oh, K Kuga, K Ishige, S Togashi, T Yanagawa, J Shoda
    Sci Rep, 2017;7(1):11977.
    Species: Mouse
    Sample Type: Serum
  7. Loss of Hepatic Mitochondrial Long-Chain Fatty Acid Oxidation Confers Resistance to Diet-Induced Obesity and Glucose Intolerance
    Authors: J Lee, J Choi, ES Selen Alpe, L Zhao, T Hartung, S Scafidi, RC Riddle, MJ Wolfgang
    Cell Rep, 2017;20(3):655-667.
    Species: Mouse
    Sample Type: Serum
  8. CD4+ T lymphocytes produce adiponectin in response to transplants
    Authors: S Danturti, KS Keslar, LR Steinhoff, R Fan, N Dvorina, A Valujskikh, RL Fairchild, WM Baldwin
    JCI Insight, 2017;2(12):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  9. Neuroendocrine androgen action is a key extraovarian mediator in the development of polycystic ovary syndrome
    Authors: AS Caldwell, MC Edwards, R Desai, M Jimenez, RB Gilchrist, DJ Handelsman, KA Walters
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Serum
  10. Mouse P2Y4 nucleotide receptor is a negative regulator of cardiac adipose-derived stem cell differentiation and cardiac fat formation
    Stem Cells Dev., 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  11. Is Western Diet-Induced Nonalcoholic Steatohepatitis in Ldlr-/- Mice Reversible?
    Authors: Lytle K, Jump D
    PLoS ONE, 2016;11(1):e0146942.
    Species: Mouse
    Sample Type: Plasma
  12. Dietary ?-linolenic acid-rich flaxseed oil prevents against alcoholic hepatic steatosis via ameliorating lipid homeostasis at adipose tissue-liver axis in mice
    Sci Rep, 2016;6(0):26826.
    Species: Mouse
    Sample Type: Plasma
  13. In vitro and in vivo analyses reveal profound effects of fibroblast growth factor 16 as a metabolic regulator
    Authors: Ingrid C Rulifson
    J. Biol. Chem, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  14. Gamma-tocotrienol attenuates high-fat diet-induced obesity and insulin resistance by inhibiting adipose inflammation and M1 macrophage recruitment.
    Authors: Zhao L, Kang I, Fang X, Wang W, Lee M, Hollins R, Marshall M, Chung S
    Int J Obes (Lond), 2015;39(3):438-46.
    Species: Mouse
    Sample Type: Plasma
  15. Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance.
    Authors: Sasaki Y, Ohta M, Desai D, Figueiredo J, Whelan M, Sugano T, Yamabi M, Yano W, Faits T, Yabusaki K, Zhang H, Mlynarchik A, Inoue K, Mizuno K, Aikawa M
    PLoS ONE, 2015;10(7):e0131176.
    Species: Mouse
    Sample Type: Plasma
  16. Additive regulation of adiponectin expression by the mediterranean diet olive oil components oleic Acid and hydroxytyrosol in human adipocytes.
    Authors: Scoditti E, Massaro M, Carluccio M, Pellegrino M, Wabitsch M, Calabriso N, Storelli C, De Caterina R
    PLoS ONE, 2015;10(6):e0128218.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  17. Docosahexaenoic acid decreases pro-inflammatory mediators in an in vitro murine adipocyte macrophage co-culture model.
    Authors: de Boer A, Monk J, Robinson L
    PLoS ONE, 2014;9(1):e85037.
    Species: Mouse
    Sample Type: Whole Cells
  18. PKClambda haploinsufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes.
    Authors: Sajan M, Ivey R, Lee M, Mastorides S, Jurczak M, Samuels V, Shulman G, Braun U, Leitges M, Farese R
    Mol Endocrinol, 2014;28(7):1097-107.
    Species: Mouse
    Sample Type: Serum
  19. Beneficial metabolic effects of rapamycin are associated with enhanced regulatory cells in diet-induced obese mice.
    Authors: Makki K, Taront S, Molendi-Coste O, Bouchaert E, Neve B, Eury E, Lobbens S, Labalette M, Duez H, Staels B, Dombrowicz D, Froguel P, Wolowczuk I
    PLoS ONE, 2014;9(4):e92684.
    Species: Mouse
    Sample Type: Serum
  20. Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cbeta in mice.
    Authors: Rao X, Zhong J, Xu X, Jordan B, Maurya S, Braunstein Z, Wang T, Huang W, Aggarwal S, Periasamy M, Rajagopalan S, Mehta K, Sun Q
    PLoS ONE, 2013;8(12):e81364.
    Species: Mouse
    Sample Type: Plasma
  21. Obesity in mice with adipocyte-specific deletion of clock component Arntl.
    Authors: Paschos, Georgios, Ibrahim, Salam, Song, Wen-Lian, Kunieda, Takeshig, Grant, Gregory, Reyes, Teresa M, Bradfield, Christop, Vaughan, Cheryl H, Eiden, Michael, Masoodi, Mojgan, Griffin, Julian L, Wang, Fenfen, Lawson, John A, Fitzgerald, Garret A
    Nat Med, 2012;18(12):1768-77.
    Species: Mouse
    Sample Type: Plasma
  22. HCV core-induced nonobese hepatic steatosis is associated with hypoadiponectinemia and is ameliorated by adiponectin administration.
    Authors: Chang ML, Yeh HC, Tsou YK
    Obesity (Silver Spring), 2012;20(7):1474-80.
    Species: Mouse
    Sample Type: Serum
  23. Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice.
    Mol. Cell. Endocrinol., 2012;358(1):127-34.
    Species: Mouse
    Sample Type: Serum
  24. Disruption of the chemokine-like receptor-1 (CMKLR1) gene is associated with reduced adiposity and glucose intolerance.
    Authors: Ernst MC, Haidl ID, Zúñiga LA, Dranse HJ, Rourke JL, Zabel BA, Butcher EC, Sinal CJ
    Endocrinology, 2012;153(2):672-82.
    Species: Mouse
    Sample Type: Serum
  25. Castration-induced changes in mouse epididymal white adipose tissue.
    Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
    Mol. Cell. Endocrinol., 2011;345(1):58-67.
    Species: Mouse
    Sample Type: Serum
  26. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.
    Authors: Sato H, Isogai Y, Masuda S
    J. Biol. Chem., 2011;286(13):11632-48.
    Species: Mouse
    Sample Type: Serum
  27. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
    Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
    Diabetes, 2010;59(4):935-46.
    Species: Mouse
    Sample Type: Plasma
  28. Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice.
    Authors: Zhu M, Hug C, Kasahara DI
    Am. J. Respir. Cell Mol. Biol., 2010;43(4):487-97.
    Species: Mouse
    Sample Type: BALF
  29. Insulin sensitizing activity of ethyl acetate fraction of Acorus calamus L. in vitro and in vivo.
    Authors: Wu HS, Zhu DF, Zhou CX, Feng CR, Lou YJ, Yang B, He QJ
    J Ethnopharmacol, 2009;123(2):288-92.
    Species: Mouse
    Sample Type: Serum
  30. The peroxisome proliferator-activated receptor gamma agonist pioglitazone improves cardiometabolic risk and renal inflammation in murine lupus.
    Authors: Zhao W, Thacker SG, Hodgin JB, Zhang H, Wang JH, Park JL, Randolph A, Somers EC, Pennathur S, Kretzler M, Brosius FC, Kaplan MJ
    J. Immunol., 2009;183(4):2729-40.
    Species: Mouse
    Sample Type: Serum
  31. n-3 fatty acids and rosiglitazone improve insulin sensitivity through additive stimulatory effects on muscle glycogen synthesis in mice fed a high-fat diet.
    Authors: Kuda O, Jelenik T, Jilkova Z, Flachs P, Rossmeisl M, Hensler M, Kazdova L, Ogston N, Baranowski M, Gorski J, Janovska P, Kus V, Polak J, Mohamed-Ali V, Burcelin R, Cinti S, Bryhn M, Kopecky J
    Diabetologia, 2009;52(5):941-51.
    Species: Mouse
    Sample Type: Plasma
  32. Hydrangeic acid from the processed leaves of Hydrangea macrophylla var. thunbergii as a new type of anti-diabetic compound.
    Authors: Zhang H, Matsuda H, Yamashita C, Nakamura S, Yoshikawa M
    Eur. J. Pharmacol., 2009;606(1):255-61.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  33. PPARgamma in the endothelium regulates metabolic responses to high-fat diet in mice.
    Authors: Kanda T, Brown JD, Orasanu G, Vogel S, Gonzalez FJ, Sartoretto J, Michel T, Plutzky J
    J. Clin. Invest., 2009;119(1):110-24.
    Species: Mouse
    Sample Type: Serum
  34. Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice.
    Authors: Hyvarinen K, Tuomainen AM, Laitinen S, Bykov IL, Tormakangas L, Lindros K, Käkelä R, Alfthan G, Salminen I, Jauhiainen M, Kovanen PT, Leinonen M, Saikku P, Pussinen PJ
    Infect. Immun., 2009;77(8):3442-9.
    Species: Mouse
    Sample Type: Serum
  35. Dose dependent development of diabetes mellitus and non-alcoholic steatohepatitis in monosodium glutamate-induced obese mice.
    Authors: Sasaki Y, Suzuki W, Shimada T, Iizuka S, Nakamura S, Nagata M, Fujimoto M, Tsuneyama K, Hokao R, Miyamoto K, Aburada M
    Life Sci., 2009;85(13):490-8.
    Species: Mouse
    Sample Type: Plasma
  36. Role and regulation of adipokines during zymosan-induced peritoneal inflammation in mice.
    Authors: Pini M, Gove ME, Sennello JA, van Baal JW, Chan L, Fantuzzi G
    Endocrinology, 2008;149(8):4080-5.
    Species: Mouse
    Sample Type: Serum
  37. Octylphenol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor and extracellular signal-regulated kinase pathways.
    Authors: Lee MJ, Lin H, Liu CW, Wu MH, Liao WJ, Chang HH, Ku HC, Chien YS, Ding WH, Kao YH
    Am. J. Physiol., Cell Physiol., 2008;294(6):C1542-51.
    Species: Mouse
    Sample Type: Serum
  38. Novel black soy peptides with antiobesity effects: activation of leptin-like signaling and AMP-activated protein kinase.
    Authors: Jang EH, Moon JS, Ko JH, Ahn CW, Lee HH, Shin JK, Park CS, Kang JH
    Int J Obes (Lond), 2008;32(7):1161-70.
    Species: Mouse
    Sample Type: Plasma
  39. Pharmacological IKK2 inhibition blocks liver steatosis and initiation of non-alcoholic steatohepatitis.
    Authors: Beraza N, Malato Y, Vander Borght S, Liedtke C, Wasmuth HE, Dreano M, De Vos R, Roskams T, Trautwein C
    Gut, 2008;57(5):655-63.
  40. Capsaicin, a spicy component of hot peppers, modulates adipokine gene expression and protein release from obese-mouse adipose tissues and isolated adipocytes, and suppresses the inflammatory responses of adipose tissue macrophages.
    Authors: Kang JH, Kim CS, Han IS, Kawada T, Yu R
    FEBS Lett., 2007;581(23):4389-96.
    Species: Mouse
    Sample Type: Tissue Homogenates
  41. Effects of pioglitazone on suppressor of cytokine signaling 3 expression: potential mechanisms for its effects on insulin sensitivity and adiponectin expression.
    Authors: Kanatani Y, Usui I, Ishizuka K, Bukhari A, Fujisaka S, Urakaze M, Haruta T, Kishimoto T, Naka T, Kobayashi M
    Diabetes, 2007;56(3):795-803.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  42. Integrating genetic and network analysis to characterize genes related to mouse weight.
    Authors: Ghazalpour A, Doss S, Zhang B, Wang S, Plaisier C, Castellanos R, Brozell A, Schadt EE, Drake TA, Lusis AJ, Horvath S
    PLoS Genet., 2006;2(8):e130.
    Species: Mouse
    Sample Type: Plasma
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