Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ameloblastin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Ameloblastin
Val27-Pro407
Accession # O55189

Specificity

Detects mouse Ameloblastin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Ameloblastin Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Ameloblastin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ameloblastin

Ameloblastin, also known as amelin and sheathlin, is a 66 kDa secreted tooth-specific glycoprotein. It is a major enamel matrix protein that promotes enamel formation in teeth. Ameloblastin is secreted primarily by secretory ameloblasts. It is a cell adhesion molecule required for amelogenesis. In addition to the isoform used as immunogen, a second variant with a 15 aa insertion between Q113 and P114 (AAH 87927) is known. The amino acid sequence of mature mouse ameloblastin is 96% and 67% identical to that of rat and human ameloblastin, respectively.

Alternate Names

AMBN

Entrez Gene IDs

258 (Human); 11698 (Mouse)

Gene Symbol

AMBN

UniProt

O55189

Additional Ameloblastin Products

Product Documents for Mouse Ameloblastin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Ameloblastin Antibody

For research use only

Citations for Mouse Ameloblastin Antibody

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