Mouse Ameloblastin Antibody

Catalog #: AF3026
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3026
AF3026-SP

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Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Ameloblastin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Ameloblastin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Ameloblastin
Val27-Pro407
Accession # O55189

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Ameloblastin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ameloblastin

Ameloblastin, also known as amelin and sheathlin, is a 66 kDa secreted tooth-specific glycoprotein. It is a major enamel matrix protein that promotes enamel formation in teeth. Ameloblastin is secreted primarily by secretory ameloblasts. It is a cell adhesion molecule required for amelogenesis. In addition to the isoform used as immunogen, a second variant with a 15 aa insertion between Q113 and P114 (AAH 87927) is known. The amino acid sequence of mature mouse ameloblastin is 96% and 67% identical to that of rat and human ameloblastin, respectively.

Entrez Gene IDs

258 (Human); 11698 (Mouse)

Alternate Names

AI1F; AMBN; Ameloblastin

Product Datasheets

Product Datasheet

COA

SDS

Citation for Mouse Ameloblastin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling.
Authors: Takahashi S, Kawashima N, Sakamoto K, Nakata A, Kameda T, Sugiyama T, Katsube K, Suda H
Biochem. Biophys. Res. Commun., 2007;353(2):405-11.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot