Mouse Axl Antibody

Catalog # Availability Size / Price Qty
MAB854
MAB854-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)

Mouse Axl Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Axl in ELISAs and Western blots. Does not cross-react with recombinant human (rh) Axl, rmDtk, or rmMer.
Source
Monoclonal Rat IgG1 Clone # 107332
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Axl
His20-Pro443
Accession # Q00993
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Axl Fc Chimera (Catalog # 854-AX)

Mouse Axl Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Axl Biotinylated Antibody (Catalog # BAF854)

Standard: Recombinant Mouse Axl Fc Chimera Protein, CF (Catalog # 854-AX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Product Datasheets

Citations for Mouse Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  2. Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
    Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
    Immunology, 2012;135(1):40-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells.
    Authors: Shang T, Zhang X, Wang T, Sun B, Deng T, Han D
    Endocrinology, 2011;152(7):2827-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Sertoli cell-initiated testicular innate immune response through toll-like receptor-3 activation is negatively regulated by Tyro3, Axl, and mer receptors.
    Authors: Sun B, Qi N, Shang T
    Endocrinology, 2010;151(6):2886-97.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Axl Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
66.67%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
33.33%

Have you used Mouse Axl Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse Axl Antibody
By Anonymous on 02/24/2020
Application: WB Sample Tested: Adult kidney,Adult brain,Liver tissue,Spleen tissue Species: Mouse

Mouse Axl Antibody
By Anonymous on 04/12/2018
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Staining on live cells with MAB854 and a secondary antibody.


Mouse Axl Antibody
By Anonymous on 03/15/2018
Application: Flow Sample Tested: Mouse cell line Species: Mouse