Mouse Axl Antibody

Catalog # Availability Size / Price Qty
AF854
AF854-SP
Mouse Axl Antibody in Data
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (5)

Mouse Axl Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Axl in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Axl is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Axl
His20-Pro443
Accession # Q80YQ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Axl Fc Chimera (Catalog # 854-AX)
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Axl in Mouse Kidney. View Larger

Axl in Mouse Kidney. Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Product Datasheets

Citations for Mouse Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. YAP1/TAZ drives ependymoma-like tumour formation in mice
    Authors: N Eder, F Roncaroli, MC Dolmart, S Horswell, F Andreiuolo, HR Flynn, AT Lopes, S Claxton, JP Kilday, L Collinson, JH Mao, T Pietsch, B Thompson, AP Snijders, SK Ultanir
    Nat Commun, 2020;11(1):2380.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Epigenetic regulation of brain region-specific microglia clearance activity
    Authors: P Ayata, A Badimon, HJ Strasburge, MK Duff, SE Montgomery, YE Loh, A Ebert, AA Pimenova, BR Ramirez, AT Chan, JM Sullivan, I Purushotha, JR Scarpa, AM Goate, M Busslinger, L Shen, B Losic, A Schaefer
    Nat. Neurosci., 2018;21(8):1049-1060.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
    Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
    Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
    Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
    Neuron, 2017;94(3):500-516.e9.
  7. MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis
    Authors: M Kurowska-S, S Alivernini, EG Melchor, A Elmesmari, B Tolusso, C Tange, L Petricca, DS Gilchrist, G Di Sante, C Keijzer, L Stewart, C Di Mario, V Morrison, JM Brewer, D Porter, S Milling, RD Baxter, D McCarey, E Gremese, G Lemke, G Ferracciol, C McSharry, IB McInnes
    Nat Commun, 2017;8(0):15877.
  8. TAM receptors regulate multiple features of microglial physiology
    Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
    Nature, 2016;532(7598):240-4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Opposing Roles of Tyrosine Kinase Receptors Mer and Axl Determine Clinical Outcomes in Experimental Immune-Mediated Nephritis
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014;15(10):920-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  11. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
    Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
    J. Neurosci., 2010;30(46):15521-34.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Secondary Antibodies

Reviews for Mouse Axl Antibody

Average Rating: 4 (Based on 5 Reviews)

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Mouse Axl Antibody
By Anonymous on 11/19/2019
Application: IHC Sample Tested: Cancer Tissue Species: Mouse

Stained FFPE tissue sections from mouse lung tumors with 1:20 and 1:100 dilutions of AF854, detected with DAB chromagen.


Mouse Axl Antibody
By Anonymous on 02/19/2019
Application: WB Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

The first lane is a wild type line, and the next to are shRNA Axl knockdowns


Mouse Axl Antibody
By Anonymous on 01/04/2019
Application: WB Sample Tested: Bone marrow cells Species: Mouse

Mouse Axl Antibody
By Anonymous on 06/22/2017
Application: IHC Sample Tested: Brain (cerebral cortex) Species: Mouse

Mouse Axl Antibody
By Anonymous on 06/07/2017
Application: Func Sample Tested: Spinal cord,Brain tissue Species: Mouse