Mouse Axl Antibody
Mouse Axl Antibody Summary
His20-Pro443
Accession # Q80YQ3
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Axl in Mouse Kidney. Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
Product Datasheets
Citations for Mouse Axl Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL
Authors: A Ko, M Hasanain, YT Oh, F D'Angelo, D Sommer, B Frangaj, S Tran, F Bielle, B Pollo, R Paterra, K Mokhtari, RK Soni, M Peyre, M Eoli, L Papi, M Kalamaride, M Sanson, A Iavarone, A Lasorella
Cancer Discovery, 2022;0(0):.
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop
Authors: Y Shen, Q Zhu, M Xiao, L Yin, W Feng, J Feng, J He, P Li, X Chen, W Ding, J Zhong, Z Zeng, Z Xie, J Liu, X Zu
Cell Death & Disease, 2022;13(8):749.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
Nature Medicine, 2022;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Embryonic Microglia Interact with Hypothalamic Radial Glia during Development and Upregulate the TAM Receptors MERTK and AXL following an Insult
Authors: JM Rosin, CM Marsters, F Malik, R Far, L Adnani, C Schuurmans, QJ Pittman, DM Kurrasch
Cell Reports, 2021;34(1):108587.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
Cell Death & Disease, 2021;12(6):538.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Microglia use TAM receptors to detect and engulf amyloid &beta plaques
Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
Nature Immunology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
YAP1/TAZ drives ependymoma-like tumour formation in mice
Authors: N Eder, F Roncaroli, MC Dolmart, S Horswell, F Andreiuolo, HR Flynn, AT Lopes, S Claxton, JP Kilday, L Collinson, JH Mao, T Pietsch, B Thompson, AP Snijders, SK Ultanir
Nat Commun, 2020;11(1):2380.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain
Authors: C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé
Nature Communications, 2020;11(1):6133.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis
Authors: JC DuBois, AK Ray, P Davies, B Shafit-Zag
J Neuroinflammation, 2020;17(1):324.
Species: Mouse
Sample Types: In Vivo
Applications: Functional Assay -
Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS
Authors: LA Hohsfield, AR Najafi, Y Ghorbanian, N Soni, EE Hingco, SJ Kim, AD Jue, V Swarup, MA Inlay, KN Green
Journal of Neuroinflammation, 2020;17(1):279.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
AXL targeting reduces fibrosis development in experimental unilateral ureteral obstruction
Authors: L Landolt, J Furriol, J Babickova, L Ahmed, Ø Eikrem, T Skogstrand, A Scherer, S Suliman, S Leh, JB Lorens, G Gausdal, HP Marti, T Osman
Physiol Rep, 2019;7(10):e14091.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Epigenetic regulation of brain region-specific microglia clearance activity
Authors: P Ayata, A Badimon, HJ Strasburge, MK Duff, SE Montgomery, YE Loh, A Ebert, AA Pimenova, BR Ramirez, AT Chan, JM Sullivan, I Purushotha, JR Scarpa, AM Goate, M Busslinger, L Shen, B Losic, A Schaefer
Nat. Neurosci., 2018;21(8):1049-1060.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis
Authors: M Kurowska-S, S Alivernini, EG Melchor, A Elmesmari, B Tolusso, C Tange, L Petricca, DS Gilchrist, G Di Sante, C Keijzer, L Stewart, C Di Mario, V Morrison, JM Brewer, D Porter, S Milling, RD Baxter, D McCarey, E Gremese, G Lemke, G Ferracciol, C McSharry, IB McInnes
Nat Commun, 2017;8(0):15877.
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Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
Neuron, 2017;94(3):500-516.e9.
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Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Opposing Roles of Tyrosine Kinase Receptors Mer and Axl Determine Clinical Outcomes in Experimental Immune-Mediated Nephritis
J Immunol, 2016;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
TAM receptors regulate multiple features of microglial physiology
Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
Nature, 2016;532(7598):240-4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Diversification of TAM receptor tyrosine kinase function.
Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
Nat Immunol, 2014;15(10):920-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
J. Exp. Med., 2012;209(11):2033-47.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
J. Neurosci., 2010;30(46):15521-34.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
Authors: Zhu D, Wang Y, Singh I
Blood, 2010;115(23):4963-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
J. Thromb. Haemost., 2005;3(4):733-41.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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Stained FFPE tissue sections from mouse lung tumors with 1:20 and 1:100 dilutions of AF854, detected with DAB chromagen.
The first lane is a wild type line, and the next to are shRNA Axl knockdowns
