Mouse Axl Antibody

Catalog # Availability Size / Price Qty
Axl in Mouse Kidney.
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Product Details
Citations (24)
Supplemental Products
Reviews (5)

Mouse Axl Antibody Summary

Species Reactivity
Detects mouse Axl in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Axl is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Axl
Accession # Q80YQ3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Axl Fc Chimera (Catalog # 854-AX)
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Axl antibody in Mouse Kidney by Immunohistochemistry (IHC-P). View Larger

Axl in Mouse Kidney. Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

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Citations for Mouse Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL
    Authors: A Ko, M Hasanain, YT Oh, F D'Angelo, D Sommer, B Frangaj, S Tran, F Bielle, B Pollo, R Paterra, K Mokhtari, RK Soni, M Peyre, M Eoli, L Papi, M Kalamaride, M Sanson, A Iavarone, A Lasorella
    Cancer Discovery, 2022;0(0):.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop
    Authors: Y Shen, Q Zhu, M Xiao, L Yin, W Feng, J Feng, J He, P Li, X Chen, W Ding, J Zhong, Z Zeng, Z Xie, J Liu, X Zu
    Cell Death & Disease, 2022;13(8):749.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
    Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
    Nature Medicine, 2022;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Embryonic Microglia Interact with Hypothalamic Radial Glia during Development and Upregulate the TAM Receptors MERTK and AXL following an Insult
    Authors: JM Rosin, CM Marsters, F Malik, R Far, L Adnani, C Schuurmans, QJ Pittman, DM Kurrasch
    Cell Reports, 2021;34(1):108587.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
    Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
    Cell Death & Disease, 2021;12(6):538.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Microglia use TAM receptors to detect and engulf amyloid &beta plaques
    Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
    Nature Immunology, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. YAP1/TAZ drives ependymoma-like tumour formation in mice
    Authors: N Eder, F Roncaroli, MC Dolmart, S Horswell, F Andreiuolo, HR Flynn, AT Lopes, S Claxton, JP Kilday, L Collinson, JH Mao, T Pietsch, B Thompson, AP Snijders, SK Ultanir
    Nat Commun, 2020;11(1):2380.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain
    Authors: C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé
    Nature Communications, 2020;11(1):6133.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  9. Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis
    Authors: JC DuBois, AK Ray, P Davies, B Shafit-Zag
    J Neuroinflammation, 2020;17(1):324.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Functional Assay
  10. Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS
    Authors: LA Hohsfield, AR Najafi, Y Ghorbanian, N Soni, EE Hingco, SJ Kim, AD Jue, V Swarup, MA Inlay, KN Green
    Journal of Neuroinflammation, 2020;17(1):279.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. AXL targeting reduces fibrosis development in experimental unilateral ureteral obstruction
    Authors: L Landolt, J Furriol, J Babickova, L Ahmed, Ø Eikrem, T Skogstrand, A Scherer, S Suliman, S Leh, JB Lorens, G Gausdal, HP Marti, T Osman
    Physiol Rep, 2019;7(10):e14091.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Epigenetic regulation of brain region-specific microglia clearance activity
    Authors: P Ayata, A Badimon, HJ Strasburge, MK Duff, SE Montgomery, YE Loh, A Ebert, AA Pimenova, BR Ramirez, AT Chan, JM Sullivan, I Purushotha, JR Scarpa, AM Goate, M Busslinger, L Shen, B Losic, A Schaefer
    Nat. Neurosci., 2018;21(8):1049-1060.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis
    Authors: M Kurowska-S, S Alivernini, EG Melchor, A Elmesmari, B Tolusso, C Tange, L Petricca, DS Gilchrist, G Di Sante, C Keijzer, L Stewart, C Di Mario, V Morrison, JM Brewer, D Porter, S Milling, RD Baxter, D McCarey, E Gremese, G Lemke, G Ferracciol, C McSharry, IB McInnes
    Nat Commun, 2017;8(0):15877.
  15. Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
    Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
    Neuron, 2017;94(3):500-516.e9.
  16. Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
    Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
    Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. Opposing Roles of Tyrosine Kinase Receptors Mer and Axl Determine Clinical Outcomes in Experimental Immune-Mediated Nephritis
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. TAM receptors regulate multiple features of microglial physiology
    Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
    Nature, 2016;532(7598):240-4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014;15(10):920-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  21. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
    Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
    J. Neurosci., 2010;30(46):15521-34.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Mouse Axl Antibody
By Tara Peters on 11/19/2019
Application: IHC Sample Tested: Cancer Tissue Species: Mouse

Stained FFPE tissue sections from mouse lung tumors with 1:20 and 1:100 dilutions of AF854, detected with DAB chromagen.

Mouse Axl Antibody
By Anonymous on 02/19/2019
Application: WB Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

The first lane is a wild type line, and the next to are shRNA Axl knockdowns

Mouse Axl Antibody
By Anonymous on 01/04/2019
Application: WB Sample Tested: Bone marrow cells Species: Mouse

Mouse Axl Antibody
By Marco Onorati on 06/22/2017
Application: IHC Sample Tested: Brain (cerebral cortex) Species: Mouse

Mouse Axl Antibody
By Anonymous on 06/07/2017
Application: Func Sample Tested: Brain tissue,Spinal cord Species: Mouse