Mouse B220/CD45R MAb (Clone RA3-6B2)

MLDP7 has been discontinued and is replaced by MAB1217.
    
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  • Species Reactivity
    Mouse
  • Specificity
    This antibody has been shown to react with the extracellular domains of a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells.1 - 3 The level of B220 antigen expression on the B cell lineage is developmentally regulated 2 - 4 and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses.5, 6
  • Source
    Monoclonal Rat IgG2a Clone # RA3-6B2
  • Label
    Unconjugated
Product Datasheets

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Applications
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    Recommended
    Concentration
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  • Cell depletion
    This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete B220+ cells from 1 x 109 bone marrow cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Depletion

Figure 1: Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Cell Depletion
Cell Depletion
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Stability & Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: B220/CD45R
B220 antigens represent a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on the B cell lineage is developmentally regulated and this antibody is commonly used as a B cell marker.
  • References:
    1. Current Protocols in Immunology, Isolation of Murine Macrophages, copyright 1994, Coligan, J.E. et al. eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 24699 (Rat)
  • Alternate Names:
    B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
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