Mouse B220/CD45R MAb (Clone RA3-6B2)

Name: Mouse B220/CD45R MAb (Clone RA3-6B2)
Brand: R&D Systems
SKU: MLDP7

Catalog #: MLDP7 Datasheet / COA / SDS

Newer Version Available: MAB1217

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Discontinued Product

MLDP7 has been discontinued and is replaced by MAB1217.

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All B220/CD45R Antibodies

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Mouse B220/CD45R MAb (Clone RA3-6B2) Summary

Species Reactivity

Mouse

Specificity

This antibody has been shown to react with the extracellular domains of a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells.^{1 - 3} The level of B220 antigen expression on the B cell lineage is developmentally regulated ^{2 - 4} and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses.^{5, 6}

Source

Monoclonal Rat IgG2a Clone # RA3-6B2

Label

Unconjugated

Applications

Recommended Concentration

Sample

Cell depletion

This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete B220+ cells from 1 x 10⁹ bone marrow cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion

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Cell Depletion

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Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Cell Depletion

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Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: B220/CD45R

B220 antigens represent a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on the B cell lineage is developmentally regulated and this antibody is commonly used as a B cell marker.

References

Current Protocols in Immunology, Isolation of Murine Macrophages, copyright 1994, Coligan, J.E. et al. eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.

Long Name

Cluster of Differentiation 45

Entrez Gene IDs

5788 (Human); 19264 (Mouse); 24699 (Rat)

Alternate Names

B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide