Mouse B220/CD45R Antibody

Catalog # Availability Size / Price Qty
MAB1217
MAB1217-SP

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B220/CD45R in Mouse Splenocytes.
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Product Details
Citations (10)
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Mouse B220/CD45R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R.
Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
Coffman, R.L. (1982) Immuno. Rev. 69:5.
 
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry B220/CD45R antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References
  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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Citations for Mouse B220/CD45R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction
    Authors: Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA;
    Journal of the American Heart Association
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Fine-tuning of MEK signaling is pivotal for limiting B and T�cell activation
    Authors: N Houde, L Beuret, A Bonaud, SP Fortier-Be, K Truchon-La, R Aoidi, É Pic, N Alouche, V Rondeau, G Schlecht-L, K Balabanian, M Espéli, J Charron
    Cell Reports, 2022-01-11;38(2):110223.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Kindlin-3 maintains marginal zone B cells but confines follicular B cell activation and differentiation
    Authors: A Härzschel, L Li, PW Krenn, E Szenes-Nag, G Andrieux, E Bayer, D Pfeifer, L Polcik, U Denk, JP Höpner, E Karabatak, DJ Danner, S Tangermann, L Kenner, H Jumaa, R Greil, M Börries, R Ruppert, PC Maity, TN Hartmann
    Journal of leukocyte biology, 2021-12-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A
    Authors: R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu
    Theranostics, 2021-01-01;11(1):117-131.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Combined immunotherapy with anti-PDL-1/PD-1 and anti-CD4 antibodies cures syngeneic disseminated neuroblastoma
    Authors: V Rigo, L Emionite, A Daga, S Astigiano, MV Corrias, C Quintarell, F Locatelli, S Ferrini, M Croce
    Sci Rep, 2017-10-25;7(1):14049.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
    Sci Rep, 2016-08-22;6(0):31874.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016-08-15;18(9):979-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. B-cell-derived IL-10 does not vitally contribute to the clinical course of glomerulonephritis.
    Authors: Kluger M, Ostmann A, Luig M, Meyer M, Goerke B, Paust H, Meyer-Schwesinger C, Stahl R, Panzer U, Tiegs G, Steinmetz O
    Eur J Immunol, 2013-12-16;44(3):683-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008-10-16;382(1):20-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Small changes in lymphocyte development and activation in mice through tissue-specific alteration of heparan sulphate.
    Authors: Garner OB, Yamaguchi Y, Esko JD, Videm V
    Immunology, 2008-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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