Mouse B220/CD45R Antibody
Mouse B220/CD45R Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
- Coffman, R.L. (1982) Immuno. Rev. 69:5.
- Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
- Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
- Allman, D.M. et al. (1992) J. Immunol. 149:2533.
- Asensi, V.K. et al. (1989) Immunology 68:204.
- Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Product Datasheets
Citations for Mouse B220/CD45R Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Fine-tuning of MEK signaling is pivotal for limiting B and T�cell activation
Authors: N Houde, L Beuret, A Bonaud, SP Fortier-Be, K Truchon-La, R Aoidi, É Pic, N Alouche, V Rondeau, G Schlecht-L, K Balabanian, M Espéli, J Charron
Cell Reports, 2022;38(2):110223.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A
Authors: R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu
Theranostics, 2021;11(1):117-131.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Kindlin-3 maintains marginal zone B cells but confines follicular B cell activation and differentiation
Authors: A Härzschel, L Li, PW Krenn, E Szenes-Nag, G Andrieux, E Bayer, D Pfeifer, L Polcik, U Denk, JP Höpner, E Karabatak, DJ Danner, S Tangermann, L Kenner, H Jumaa, R Greil, M Börries, R Ruppert, PC Maity, TN Hartmann
Journal of leukocyte biology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Combined immunotherapy with anti-PDL-1/PD-1 and anti-CD4 antibodies cures syngeneic disseminated neuroblastoma
Authors: V Rigo, L Emionite, A Daga, S Astigiano, MV Corrias, C Quintarell, F Locatelli, S Ferrini, M Croce
Sci Rep, 2017;7(1):14049.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
NOTCH1 mediates a switch between two distinct secretomes during senescence
Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
Nat. Cell Biol, 2016;18(9):979-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
Sci Rep, 2016;6(0):31874.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
B-cell-derived IL-10 does not vitally contribute to the clinical course of glomerulonephritis.
Authors: Kluger M, Ostmann A, Luig M, Meyer M, Goerke B, Paust H, Meyer-Schwesinger C, Stahl R, Panzer U, Tiegs G, Steinmetz O
Eur J Immunol, 2014;44(3):683-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Small changes in lymphocyte development and activation in mice through tissue-specific alteration of heparan sulphate.
Authors: Garner OB, Yamaguchi Y, Esko JD, Videm V
Immunology, 2008;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
Virology, 2008;382(1):20-7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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