Mouse B220/CD45R Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse B220/CD45R.
  • Source
    Monoclonal Rat IgG2A Clone # RA3-6B2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Abelson mouse leukemia virus-induced pre-B tumor cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation
    Coffman, R.L. (1982) Immuno. Rev. 69:5.
  • CyTOF-reported
    Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju­gated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

  • References:
    1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
    2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
    3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
    4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
    5. Asensi, V.K. et al. (1989) Immunology 68:204.
    6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 24699 (Rat)
  • Alternate Names:
    B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
    Sci Rep, 2016;6(0):31874.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016;18(9):979-92.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  3. B-cell-derived IL-10 does not vitally contribute to the clinical course of glomerulonephritis.
    Authors: Kluger M, Ostmann A, Luig M, Meyer M, Goerke B, Paust H, Meyer-Schwesinger C, Stahl R, Panzer U, Tiegs G, Steinmetz O
    Eur J Immunol, 2014;44(3):683-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Small changes in lymphocyte development and activation in mice through tissue-specific alteration of heparan sulphate.
    Authors: Garner OB, Yamaguchi Y, Esko JD, Videm V
    Immunology, 2008;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008;382(1):20-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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