Detection of Mouse Bcl‑2 by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl‑2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl‑2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Bcl-2 family of proteins plays a crucial role in the regulation of
cell death in many eukaryotic systems. Bcl-2 is a 26 kD protein
associated with cell survival. Over-expression of Bcl-2 has been shown
to promote cell survival by suppressing apoptosis in a number of cells.
The ratio of Bcl-2 to other Bcl-2 family members and subcellular
localization are believed to modulate the apoptotic process.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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