Mouse Bcl-2 Minus C-Terminus Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Bcl‑2 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Mouse Bcl-2 Minus C-Terminus Antibody Summary

Species Reactivity
Detects mouse Bcl‑2 Minus C-Terminus in Western blots.
Monoclonal Rat IgG2B Clone # 121529
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Bcl-2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse Bcl‑2 antibody by Western Blot. View Larger

Detection of Mouse Bcl‑2 by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl-2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-2

The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bcl-2 is a 26 kD protein associated with cell survival. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis in a number of cells. The ratio of Bcl-2 to other Bcl-2 family members and subcellular localization are believed to modulate the apoptotic process.

Long Name
B Cell Lymphoma/Leukemia 2
Entrez Gene IDs
596 (Human); 12043 (Mouse); 24224 (Rat)
Alternate Names
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

Product Datasheets

Citation for Mouse Bcl-2 Minus C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Bakuchiol-induced caspase-3-dependent apoptosis occurs through c-Jun NH2-terminal kinase-mediated mitochondrial translocation of Bax in rat liver myofibroblasts.
    Authors: Park EJ, Zhao YZ, Kim YC, Sohn DH
    Eur. J. Pharmacol., 2007;559(2):115-23.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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