Mouse Caspase-11/Caspase-4 Antibody

Catalog # Availability Size / Price Qty
MAB86481
MAB86481-SP
Caspase-11/Caspase-4 in Mouse Splenocytes.
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Mouse Caspase-11/Caspase-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Caspase-11/Caspase-4 in direct ELISA.
Source
Monoclonal Rat IgG2B Clone # 919916
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Caspase-11/Caspase-4
Pro81-Glu266
Accession # P70343
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Caspase-11/Caspase-4 in Mouse Splenocytes. View Larger

Caspase-11/Caspase-4 in Mouse Splenocytes. Caspase-11/Caspase-4 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB86481) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-11/Caspase-4

Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines. Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen. The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373).  Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta. Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively. 

Entrez Gene IDs
12363 (Mouse); 114555 (Rat)
Alternate Names
CASP11; Caspase-11; Caspl; Ich-3

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG2B Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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