Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Cathepsin X/Z/P Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
Ala23-Val306
Accession # Q9WUU7

Specificity

Detects mouse Cathepsin X/Z/P in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Cathepsin X/Z/P is observed and less than 2% cross-reactivity with recombinant mouse (rm) Cathepsin-D, rmCathepsin-H, rmCathepsin-L, rmCathepsin‑C, and rmCathepsin-B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Cathepsin X/Z/P Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References

  1. Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
  2. Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
  3. Nagler and Menard (1998) FEBS Lett. 434:135.
  4. Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
  5. Pungercar, et al. (2000) Pflugers Arch. 439:R119.
  6. Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, et al. (2001) Biol. Chem. 382:839.
  8. Therrien, et al. (2001) Biochemistry 40:2702.
  9. Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, et al. (1996) J. Biol. Chem. 271:30181.

Alternate Names

Cathepsin P, Cathepsin Z, CTSZ

Entrez Gene IDs

1522 (Human); 64138 (Mouse)

Gene Symbol

CTSZ

UniProt

Q9WUU7

Additional Cathepsin X/Z/P Products

Product Documents for Mouse Cathepsin X/Z/P Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Cathepsin X/Z/P Biotinylated Antibody

For research use only

Citations for Mouse Cathepsin X/Z/P Biotinylated Antibody

Customer Reviews for Mouse Cathepsin X/Z/P Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Cathepsin X/Z/P Biotinylated Antibody and earn rewards!

Have you used Mouse Cathepsin X/Z/P Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies