Mouse Cathepsin X/Z/P Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Cathepsin X/Z/P in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Cathepsin X/Z/P is observed and less than 2% cross-reactivity with recombinant mouse Cathepsin B, C, D, H, and L is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
    Ala23-Val306
    Accession # Q9WUU7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY), see our available Western blot detection antibodies
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Recombinant Mouse Cathepsin X/Z/P (0.1 µg/mL, Catalog # 1033-CY) cleavage of the fluorogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH (10 µM, Catalog # ES005). The Neutralization Dose (ND50) is typically 2.0 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Cathepsin X/Z/P in Mouse Splenocytes. Cathepsin X/Z/P was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1033) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

  • References:
    1. Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
    2. Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
    3. Nagler and Menard (1998) FEBS Lett. 434:135.
    4. Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
    5. Pungercar, et al. (2000) Pflugers Arch. 439:R119.
    6. Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
    7. Menard, et al. (2001) Biol. Chem. 382:839.
    8. Therrien, et al. (2001) Biochemistry 40:2702.
    9. Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
    10. Guncar, et al. (2000) Structure Fold Des. 8:305.
    11. Lustigman, et al. (1996) J. Biol. Chem. 271:30181.
  • Entrez Gene IDs:
    1522 (Human); 64138 (Mouse)
  • Alternate Names:
    Cathepsin P; Cathepsin X/Z/P; Cathepsin Z; CTSZ
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Inhibition of cathepsin X reduces the strength of microglial-mediated neuroinflammation
    Neuropharmacology, 2016;114(0):88-100.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
    Authors: Makrypidi G, Damme M, Muller-Loennies S, Trusch M, Schmidt B, Schluter H, Heeren J, Lubke T, Saftig P, Braulke T
    Mol. Cell. Biol., 2012;32(4):774-82.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of {alpha}1-antitrypsin.
    Authors: Zhang B, Zheng C, Zhu M, Tao J, Vasievich MP, Baines A, Kim J, Schekman R, Kaufman RJ, Ginsburg D
    Blood, 2011;118(12):3384-91.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009;110(6):1931-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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