Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody

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Product Details
Citations (4)
Supplemental Products
Reviews (2)

Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody Summary

Species Reactivity
Detects mouse CCL1/I-309/TCA-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human I-309, recombinant mouse (rm) Eotaxin-2, rmTARC, and rmMIP-3 beta is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CCL1/I-309/TCA-3
Accession # P10146
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse CCL1/I‑309/TCA‑3 (Catalog # 845-TC)

Mouse CCL1/TCA-3 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL1/I‑309/TCA‑3 Antibody (Catalog # MAB8451)

Standard: Recombinant Mouse CCL1/TCA-3 Protein (Catalog # 845-TC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta, and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

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Citations for Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  2. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  3. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008;122(3):617-24.e6.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  4. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Yang L, Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development


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Reviews for Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody

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Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody
By Katie Myers on 11/29/2022
Application: ELISA Sample Tested: Serum Species: Mouse

Used as ELISA detection

Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody
By Anonymous on 01/05/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

This antibody was used as the detection molecule in an ELISA with MAB8451 as the solid phase molecule.