Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

ELISA Development, Functional Assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 148107
View all CCL1/I-309/TCA-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I‑309/TCA‑3
Lys24-Cys92
Accession # P10146

Specificity

Detects mouse CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL1, recombinant mouse (rm) CCL17, rmCCL19 or rmCCL24 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL1/I‑309/TCA‑3 Antibody

Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody.

Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody.

Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL1/I-309/ TCA-3 Monoclonal Antibody (Catalog # MAB8451). The ND50 is typically 0.6-3.0 µg/mL.

Applications for Mouse CCL1/I‑309/TCA‑3 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL1/I‑309/TCA‑3.

Mouse CCL1/TCA-3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF845)
  • Standard: Recombinant Mouse CCL1/TCA-3 Protein (Catalog # 845-TC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB8451 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL1/I-309/TCA-3

Mouse TCA‑3 is a member of the CC beta family of chemokines, designated CCL1. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, is the orthologue of mouse TCA‑3. Mouse TCA‑3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA‑3 cDNA encodes a 92 aa precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA‑3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA‑3 has been shown to chemoattract T-cells.

References

  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.
 

Alternate Names

I-309, P500, SCYA1, SCYA2, TCA-3, TCA3

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Gene Symbol

CCL1

UniProt

P10146

Additional CCL1/I-309/TCA-3 Products

Product Documents for Mouse CCL1/I‑309/TCA‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL1/I‑309/TCA‑3 Antibody

For research use only

Citations for Mouse CCL1/I‑309/TCA‑3 Antibody

Customer Reviews for Mouse CCL1/I‑309/TCA‑3 Antibody (2)

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2 Customer Ratings
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Customer Images

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  • Mouse CCL1/I-309/TCA-3 Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/28/2022
    Worked as capture IgG
    Mouse CCL1/I‑309/TCA‑3 Antibody MAB8451
  • Mouse CCL1/I-309/TCA-3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Mouse
    Verified Customer | Posted 01/05/2018
    Together with BAF845 and ELISA was built to measure TCA-3 in mouse samples.
    Mouse CCL1/I‑309/TCA‑3 Antibody MAB8451

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