Mouse CCL1/I-309/TCA-3 Antibody

  (3 citations)
(1 Review)
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL1, recombinant mouse (rm) CCL17, rmCCL19 or rmCCL24 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 148107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I‑309/TCA‑3
    Lys24-Cys92
    Accession # P10146
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse CCL1/TCA-3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CCL1/I‑309/TCA‑3 Antibody (Catalog # MAB8451 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF845 )
  • ELISA Standard
     
    Recombinant Mouse CCL1/TCA-3 Protein (Catalog # 845-TC )
  • Neutralization
    Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody. Recombinant Mouse CCL1/TCA‑3 (Catalog # 845‑TC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA‑3 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL1/I-309/
TCA‑3 Monoclonal Antibody (Catalog # MAB8451). The ND50 is typically 0.6‑3.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3

Mouse TCA‑3 is a member of the CC beta family of chemokines, designated CCL1. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, is the orthologue of mouse TCA‑3. Mouse TCA‑3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA‑3 cDNA encodes a 92 aa precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA‑3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA‑3 has been shown to chemoattract T-cells.

  • References:
    1. Goya, I. et al. (1998) J. Immunol. 160:1975.
    2. Burd, P. et al. (1987) J. Immunol. 139:3126.
     
  • Entrez Gene IDs:
    6346 (Human); 20290 (Mouse)
  • Alternate Names:
    C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2015;185(2):420-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  3. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008;122(3):617-24.e6.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development

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Isotype Controls
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Rat IgG2B Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB , Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL015 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL014
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Average Rating: 4 (Based on 1 review)

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ELISA Mouse CCL1/I-309/TCA-3 Antibody MAB8451 Array MAB8451
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 ELISA Mouse Anonymous 01/05/2018
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ELISA Mouse CCL1/I-309/TCA-3 Antibody MAB8451
ELISA: Mouse CCL1/I-309/TCA-3 Antibody [MAB8451].

Summary

Application ELISA
Sample Tested Serum and Plasma
Species Mouse

Other Experimental Details

Other Experimental Details Together with BAF845 and ELISA was built to measure TCA-3 in mouse samples.

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