Mouse CCL1/I-309/TCA-3 Antibody

Catalog # Availability Size / Price Qty
MAB8451
MAB8451-SP

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Mouse CCL1/I-309/TCA-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL1, recombinant mouse (rm) CCL17, rmCCL19 or rmCCL24 is observed.
Source
Monoclonal Rat IgG2B Clone # 148107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I‑309/TCA‑3
Lys24-Cys92
Accession # P10146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Mouse CCL1/TCA-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF845)

Standard: Recombinant Mouse CCL1/TCA-3 Protein (Catalog # 845-TC)

Neutralization
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL1/I‑309/TCA‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody. View Larger

Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody. Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL1/I-309/ TCA-3 Monoclonal Antibody (Catalog # MAB8451). The ND50 is typically 0.6-3.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Mouse TCA‑3 is a member of the CC beta family of chemokines, designated CCL1. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, is the orthologue of mouse TCA‑3. Mouse TCA‑3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA‑3 cDNA encodes a 92 aa precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA‑3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA‑3 has been shown to chemoattract T-cells.

References
  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.
 
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

Product Datasheets

You must select a language.

x

Citations for Mouse CCL1/I-309/TCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2015;185(2):420-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  3. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008;122(3):617-24.e6.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse CCL1/I-309/TCA-3 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse CCL1/I-309/TCA-3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse CCL1/I-309/TCA-3 Antibody
By Anonymous on 01/05/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

Together with BAF845 and ELISA was built to measure TCA-3 in mouse samples.