Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Bioassay, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 123616
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # P10148

Specificity

Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human (rh) CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rmCCL1, 3, 4, 5, 6, 7,  9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL2/JE/MCP‑1 Antibody

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody.

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody.

Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.
Detection of Mouse CCL2/JE/MCP-1 by Immunohistochemistry

Detection of Mouse CCL2/JE/MCP-1 by Immunohistochemistry

Tryptase directly induced the expression of angiogenic factors in retinal endothelial cells.(F) Addition of recombinant mMCP6 into the culture of primary retinal endothelial cells induced typical tube formation. The effect of mMCP6 was suppressed by addition of anti-MCP1 mAbs. n = 4 in each group. *P < 0.05 versus rat isotype mAb, Dunnett’s test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28990934), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCL2/JE/MCP‑1 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

References

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

P10148

Additional CCL2/JE/MCP-1 Products

Product Documents for Mouse CCL2/JE/MCP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL2/JE/MCP‑1 Antibody

For research use only

Citations for Mouse CCL2/JE/MCP‑1 Antibody

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