Mouse CCL2/JE/MCP-1 Antibody

Catalog # Availability Size / Price Qty
Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody.
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Product Details
Citations (18)
Supplemental Products

Mouse CCL2/JE/MCP-1 Antibody Summary

Species Reactivity
Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human (rh) CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rmCCL1, 3, 4, 5, 6, 7,  9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.
Monoclonal Rat IgG2B Clone # 123616
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse CCL2/JE/MCP-1
Accession # P10148
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. View Larger

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.
Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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Citations for Mouse CCL2/JE/MCP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression
    Authors: X Li, B Yuan, M Lu, Y Wang, N Ding, C Liu, M Gao, Z Yao, S Zhang, Y Zhao, L Xie, Z Chen
    Nature Communications, 2021;12(1):7213.
    Species: Transgenic Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury
    Authors: L Li, L Ni, RF Heary, S Elkabes
    J Neuroinflammation, 2020;17(1):73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Keratinocyte-Macrophage Crosstalk by the Nrf2/Ccl2/EGF Signaling Axis Orchestrates Tissue Repair
    Authors: A Villarreal, MW Tiruneh, J Lee, CF Guerrero-J, J Kuhn, JA David, K Dammeyer, R Mc Kell, J Kwong, PS Rabbani, Q Nie, DJ Ceradini
    Cell Rep, 2020;33(8):108417.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids
    Authors: RW Jenkins, AR Aref, PH Lizotte, E Ivanova, S Stinson, CW Zhou, M Bowden, J Deng, H Liu, D Miao, MX He, W Walker, G Zhang, T Tian, C Cheng, Z Wei, S Palakurthi, M Bittinger, H Vitzthum, JW Kim, A Merlino, M Quinn, C Venkataram, JA Kaplan, A Portell, PC Gokhale, B Phillips, A Smart, A Rotem, RE Jones, L Keogh, M Anguiano, L Stapleton, Z Jia, M Barzily-Ro, I Cañadas, TC Thai, MR Hammond, R Vlahos, ES Wang, H Zhang, S Li, GJ Hanna, W Huang, MP Hoang, A Piris, JP Eliane, AO Stemmer-Ra, L Cameron, MJ Su, P Shah, B Izar, M Thakuria, NR LeBoeuf, G Rabinowits, V Gunda, S Parangi, JM Cleary, BC Miller, S Kitajima, R Thummalapa, B Miao, TU Barbie, V Sivathanu, J Wong, WG Richards, R Bueno, CH Yoon, J Miret, M Herlyn, LA Garraway, EM Van Allen, GJ Freeman, PT Kirschmeie, JH Lorch, PA Ott, FS Hodi, KT Flaherty, RD Kamm, GM Boland, KK Wong, D Dornan, CP Paweletz, DA Barbie
    Cancer Discov, 2017;0(0):.
    Species: Human
    Sample Types: Organoids
    Applications: Neutralization
  6. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
    Nat Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Types: In Vivo
    Applications: IHC-Fr
  8. Near-infrared laser adjuvant for influenza vaccine.
    Authors: Kashiwagi, Satoshi, Yuan, Jianping, Forbes, Benjamin, Hibert, Mathew L, Lee, Eugene L, Whicher, Laura, Goudie, Calum, Yang, Yuan, Chen, Tao, Edelblute, Beth, Collette, Brian, Edington, Laurel, Trussler, James, Nezivar, Jean, Leblanc, Pierre, Bronson, Roderick, Tsukada, Kosuke, Suematsu, Makoto, Dover, Jeffrey, Brauns, Timothy, Gelfand, Jeffrey, Poznansky, Mark C
    PLoS ONE, 2013;8(12):e82899.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
    Authors: Scalfone, Lisa K, Nel, Hendrik, Gagliardo, Lucille, Cameron, Jody L, Al-Shokri, Shaikha, Leifer, Cynthia, Fallon, Padraic, Appleton, Judith A
    Infect Immun, 2013;81(4):1354-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization
  10. Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1.
    Authors: Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T
    Diabetes, 2012;61(6):1552-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012;189(5):2545-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
    Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
    J. Biol. Chem., 2011;286(23):20991-1001.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
    Authors: Venet F, Huang X, Chung CS, Chen Y, Ayala A
    Am. J. Pathol., 2010;176(2):764-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. The role for monocyte chemoattractant protein-1 in the generation and function of memory CD8+ T cells.
    Authors: Wang T, Dai H, Wan N, Moore Y, Dai Z
    J. Immunol., 2008;180(5):2886-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation.
    Authors: Itaya M, Sakurai E, Nozaki M, Yamada K, Yamasaki S, Asai K, Ogura Y
    Invest. Ophthalmol. Vis. Sci., 2007;48(12):5677-83.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
    Authors: Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
    J. Immunol., 2006;177(4):2651-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guerin.
    Authors: Penido C, Vieira-de-Abreu A, Bozza MT, Castro-Faria-Neto HC, Bozza PT
    J. Immunol., 2003;171(12):6788-94.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization


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