Mouse CCL2/JE/MCP-1 Antibody

(14 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human (rh) CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rmCCL1, 3, 4, 5, 6, 7,  9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.
  • Source
    Monoclonal Rat IgG2B Clone # 123616
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL2/JE/MCP-1
    Gln24-Arg96
    Accession # P10148
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

  • References:
    1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
    2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
    3. Luini, W. et al. (1994) Cytokine 6:28.
  • Entrez Gene IDs:
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
  • Alternate Names:
    CCL2; MCAF; MCP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
    Nat Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Type: In Vivo
    Application: IHC Fresh
  4. Near-infrared laser adjuvant for influenza vaccine.
    Authors: Kashiwagi, Satoshi, Yuan, Jianping, Forbes, Benjamin, Hibert, Mathew L, Lee, Eugene L, Whicher, Laura, Goudie, Calum, Yang, Yuan, Chen, Tao, Edelblute, Beth, Collette, Brian, Edington, Laurel, Trussler, James, Nezivar, Jean, Leblanc, Pierre, Bronson, Roderick, Tsukada, Kosuke, Suematsu, Makoto, Dover, Jeffrey, Brauns, Timothy, Gelfand, Jeffrey, Poznansky, Mark C
    PLoS ONE, 2013;8(12):e82899.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
    Authors: Scalfone, Lisa K, Nel, Hendrik, Gagliardo, Lucille, Cameron, Jody L, Al-Shokri, Shaikha, Leifer, Cynthia, Fallon, Padraic, Appleton, Judith A
    Infect Immun, 2013;81(4):1354-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
  6. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012;189(5):2545-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  7. Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1.
    Authors: Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T
    Diabetes, 2012;61(6):1552-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  8. Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
    Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
    J. Biol. Chem., 2011;286(23):20991-1001.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  9. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
    Authors: Venet F, Huang X, Chung CS, Chen Y, Ayala A
    Am. J. Pathol., 2010;176(2):764-73.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. The role for monocyte chemoattractant protein-1 in the generation and function of memory CD8+ T cells.
    Authors: Wang T, Dai H, Wan N, Moore Y, Dai Z
    J. Immunol., 2008;180(5):2886-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation.
    Authors: Itaya M, Sakurai E, Nozaki M, Yamada K, Yamasaki S, Asai K, Ogura Y
    Invest. Ophthalmol. Vis. Sci., 2007;48(12):5677-83.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
    Authors: Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
    J. Immunol., 2006;177(4):2651-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guerin.
    Authors: Penido C, Vieira-de-Abreu A, Bozza MT, Castro-Faria-Neto HC, Bozza PT
    J. Immunol., 2003;171(12):6788-94.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 14 Citations
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