Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 68706
View all CCL27/CTACK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL27/CTACK
Leu26-Asn120
Accession # NP_035466

Specificity

Detects mouse CCL27/CTACK in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CCL27/CTACK Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse skin

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL27/CTACK (Catalog # 725-CK)

Reviewed Applications

Read 1 review rated 5 using MAB7251 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL27/CTACK

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively (2). It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).

References

  1. Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
  2. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 258:737.
  3. Gortz, A. et al. (2002) J. Immunol. 169:1387.
  4. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
  5. Homey, B. et al. (2000) J. Immunol. 164:3465.
  6. Homey, B. et al. (2002) Nat. Med. 8:157.
  7. Inokuma, D. et al. (2006) Stem Cells 24:2810.
  8. Jarmin, D. et al. (2000) J. Immunol. 164:3460.
  9. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  10. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
  11. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
  12. Soler, D. et al. (2003) Blood 101:1677.
  13. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
  14. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
  15. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Alternate Names

CTACK, ILC

Entrez Gene IDs

10850 (Human); 20301 (Mouse); 362505 (Rat)

Gene Symbol

CCL27

UniProt

NP_035466

Additional CCL27/CTACK Products

Product Documents for Mouse CCL27/CTACK Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL27/CTACK Antibody

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse CCL27/CTACK Antibody

Customer Reviews for Mouse CCL27/CTACK Antibody (1)

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  • Mouse CCL27/CTACK Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skin tissue
    Species: Mouse
    Verified Customer | Posted 03/04/2022
    Mouse CCL27/CTACK Antibody MAB7251

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