Detection of Mouse CCR5 by Western Blot.
Western blot shows lysates of EL‑4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CCR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6138) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR5 (CC chemokine receptor 5; also CD195 and MIP-1 alpha receptor) is a 41kDa (predicted) member of the G-protein coupled receptor #1 family of proteins. It is expressed on Treg T cells, NK cells, neurons, macrophages and Th1 T cells. CCR5 responds to RANTES, MIP-1 alpha and -1 beta, and induces cell adhesion and migration. Mouse CCR5 is a 7-transmembrane glycoprotein 354 amino acids (aa) in length and it contains an O-glycosylated and sulfated extracellular N-terminus (aa 1-32), plus a phosphorylated and palmitoylated intracellular C-terminus (aa 304-354). In human and primates, CCR5 appears as a 62-64 kDa membrane form, possibly due to extensive posttranslational modification. CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Over aa 1-32, 92-104, 169-200 and 263-279 collectively, mouse CCR5 shares 88% and 78% aa identity with rat and human CCR5, respectively.
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