Mouse CD117/c-kit PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse SCF R in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 180627
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SCF R
    Gln25-Thr519 (Ala207Glu)
    Accession # P05532
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and Rat Anti-Mouse Sca‑1/Ly6 APC‑conjugated Monoclonal Antibody
(Catalog # FAB1226A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD117/c-kit
SCF R, also known as c-kit proto-oncogene protein and CD117, is a 145-160 kDa member of the type III tyrosine kinase family of proteins (1,2). It is expressed on hematopoietic and endothelial cell progenitors, mast cells, germ cells, melanocytes, trigeminal neurons, glial cells, placenta, kidney, lung, and gut. SCF R is a type I transmembrane glycoprotein that undergoes splicing during spermatogenesis. Two isoforms of 50 kDa and 30 kDa are generated that represent C-terminal intracellular functional products (3). SCF R also undergoes cleavage by select cell types, generating a 95-100 kDa soluble form (4). Over amino acids 25-519, mouse SCF R shares 73% and 87% amino acid sequence identity with human and rat SCF R, respectively.
  • References:
    1. Heldin, C-H. and J. Lennartsson (2013) Cold Spring Harb. Perspect. Biol. 5:a009100.
    2. Lennartsson, J.  and L. Ronnstrand (2012) Physil. Rev. 92:1619.
    3. Zhang, L. et al. (2013) BMC Dev. Biol. 13:38.
    4. Broudy, V.C. et al. (2001) Cytokine 15:188.
  • Long Name:
    Stem Cell Factor Receptor
  • Entrez Gene IDs:
    3815 (Human); 16590 (Mouse); 64030 (Rat)
  • Alternate Names:
    CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production.
    Authors: Tamagawa-Mineoka R, Kishida T, Mazda O
    J. Invest. Dermatol., 2011;131(7):1513-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Mobilized bone marrow progenitor cells serve as donors of cytoprotective genes for cardiac repair.
    Authors: Dai Y, Ashraf M, Zuo S, Uemura R, Dai YS, Wang Y, Haider HKh, Li T, Xu M
    J. Mol. Cell. Cardiol., 2007;44(3):607-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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