Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry.
Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and Rat Anti-Mouse Sca‑1/Ly6 APC‑conjugated Monoclonal Antibody (Catalog # FAB1226A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
SCF R, also known as c-kit proto-oncogene protein and CD117, is a 145-160 kDa member of the type III tyrosine kinase family of proteins (1,2). It is expressed on hematopoietic and endothelial cell progenitors, mast cells, germ cells, melanocytes, trigeminal neurons, glial cells, placenta, kidney, lung, and gut. SCF R is a type I transmembrane glycoprotein that undergoes splicing during spermatogenesis. Two isoforms of 50 kDa and 30 kDa are generated that represent C-terminal intracellular functional products (3). SCF R also undergoes cleavage by select cell types, generating a 95-100 kDa soluble form (4). Over amino acids 25-519, mouse SCF R shares 73% and 87% amino acid sequence identity with human and rat SCF R, respectively.
Heldin, C-H. and J. Lennartsson (2013) Cold Spring Harb. Perspect. Biol. 5:a009100.
Lennartsson, J. and L. Ronnstrand (2012) Physil. Rev. 92:1619.
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