Mouse CD117/c-kit PE-conjugated Antibody Summary
Accession # P05532
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry.
Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and Rat Anti-Mouse Sca‑1/Ly6 APC‑conjugated Monoclonal Antibody
(Catalog # FAB1226A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
SCF R, also known as c-kit proto-oncogene protein and CD117, is a 145-160 kDa member of the type III tyrosine kinase family of proteins (1,2). It is expressed on hematopoietic and endothelial cell progenitors, mast cells, germ cells, melanocytes, trigeminal neurons, glial cells, placenta, kidney, lung, and gut. SCF R is a type I transmembrane glycoprotein that undergoes splicing during spermatogenesis. Two isoforms of 50 kDa and 30 kDa are generated that represent C-terminal intracellular functional products (3). SCF R also undergoes cleavage by select cell types, generating a 95-100 kDa soluble form (4). Over amino acids 25-519, mouse SCF R shares 73% and 87% amino acid sequence identity with human and rat SCF R, respectively.
- Heldin, C-H. and J. Lennartsson (2013) Cold Spring Harb. Perspect. Biol. 5:a009100.
- Lennartsson, J. and L. Ronnstrand (2012) Physil. Rev. 92:1619.
- Zhang, L. et al. (2013) BMC Dev. Biol. 13:38.
- Broudy, V.C. et al. (2001) Cytokine 15:188.
Citations for Mouse CD117/c-kit PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production.
Authors: Tamagawa-Mineoka R, Kishida T, Mazda O
J. Invest. Dermatol., 2011;131(7):1513-20.
Sample Types: Whole Cells
Mobilized bone marrow progenitor cells serve as donors of cytoprotective genes for cardiac repair.
Authors: Dai Y, Ashraf M, Zuo S, Uemura R, Dai YS, Wang Y, Haider HKh, Li T, Xu M
J. Mol. Cell. Cardiol., 2007;44(3):607-17.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse CD117/c-kit PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Mouse CD117/c-kit PE-conjugated Antibody and earn rewards!
Have you used Mouse CD117/c-kit PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image