Mouse CD117/c-kit Antibody

Catalog # Availability Size / Price Qty
MAB1356-SP
MAB1356-500
MAB1356-100
CD117/c-kit in Mouse Brain.
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Product Details
Citations (6)
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Mouse CD117/c-kit Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD117/c-kit in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 180627
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD117/c-kit under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Lineage depleted mouse bone marrow cells
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CD117/c-kit antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

CD117/c-kit in Mouse Brain. CD117/c-kit was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # MAB1356) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to Bergmann glial cells located around Purkinje cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

SCF R is also known as the c-kit proto-oncogene protein and as CD117. Together with its ligand, stem cell factor (also called c-kit ligand or mast cell growth factor), SCF R plays an essential role in gametogenesis, melanogenesis, and hematopoiesis. SCF R is expressed in hematopoietic progenitor cells, normal B  and T lymphocyte progenitors, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF R expression has also been reported in several human tumor cell lines (1,2).

References
  1. Broudy, V. (1997) Blood 90:1345.
  2. Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citations for Mouse CD117/c-kit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Immunohistochemical Analysis of Histone H3 Modification in Newt Tail Tissue Cells following Amputation
    Authors: JW Wu, X Zhang, R Sekiya, K Aoyagi, TS Li
    Stem Cells International, 2021;2021(0):8828931.
    Species: Newt
    Sample Types: Whole Tissue
    Applications: IHC
  2. Juvenile social defeat stress exposure favors in later onset of irritable bowel syndrome-like symptoms in male mice
    Authors: K Matsumoto, K Takata, D Yamada, H Usuda, K Wada, M Tada, Y Mishima, S Ishihara, S Horie, A Saitoh, S Kato
    Scientific Reports, 2021;11(1):16276.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands
    Authors: T I, Y Sumita, T Yoshida, R Honma, M Iwatake, JLM Raudales, T Shizuno, S Kuroshima, H Masuda, M Seki, SD Tran, T Asahara, I Asahina
    Stem Cell Res Ther, 2019;10(1):304.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice
    Authors: K Kishi, A Uchida, HM Takase, H Suzuki, M Kurohmaru, N Tsunekawa, M Kanai-Azum, SA Wood, Y Kanai
    Reproduction, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell.
    Authors: Fang, Shentong, Wei, Jing, Pentinmikko, Nalle, Leinonen, Hannele, Salven, Petri
    PLoS Biol, 2012;10(10):e1001407.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
    Authors: Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(25):9530-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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