Mouse CD11b/Integrin alpha M Antibody (908649)

R&D Systems | Catalog # MAB11241

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 908649
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha M beta 2
Phe17-Asn1105 (Integrin alpha M), Gln24-Asn702 (Integrin beta 2)
Accession # NP_032427 (Integrin alpha M) & P11835 (Integrin beta 2)

Specificity

Detects recombinant mouse CD11b/Integrin alpha M in Western blot.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD11b/Integrin alpha M Antibody (908649)

Detection of Mouse CD11b/Integrin alpha M antibody by Western Blot.

Detection of Mouse CD11b/Integrin alpha M by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11b/Integrin alpha M at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD11b/Integrin alpha M antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry

Detection of CD11b/Integrin alpha M in RAW 264.7 Mouse Cell Line by Flow Cytometry

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD11b/Integrin alpha M Antibody (908649)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 Mouse Monocyte/Macrophage Cell Line

Western Blot

1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 1 review rated 3 using MAB11241 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1,2).

References

  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

NP_032427 (Integrin alpha M) & P11835 (Integrin beta 2)

Additional CD11b/Integrin alpha M Products

Product Documents for Mouse CD11b/Integrin alpha M Antibody (908649)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD11b/Integrin alpha M Antibody (908649)

For research use only

Citations for Mouse CD11b/Integrin alpha M Antibody (908649)

Customer Reviews for Mouse CD11b/Integrin alpha M Antibody (908649) (1)

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  • Mouse CD11b/Integrin alpha M Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult mouse brain tissue
    Species: Mouse
    Verified Customer | Posted 11/30/2018
    Western blot for CD11b using rat mAb MAB11241-SP in mouse brain RIPA lysate; detection using Licor Odyssey system.
    Mouse CD11b/Integrin alpha M Antibody (908649) MAB11241

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