CD229, also known as T lymphocyte surface antigen Ly-9, is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is also a member of the CD150/SLAM receptor family and is expressed on T and B lymphocytes. Mouse CD229 contains 2 Ig-like C2-type domains and 2 Ig-like V-type domains in its extracellular region. Two mouse alleles that differ in 6 extracellular amino acid residues have been reported. Human and mouse CD229 share 60% amino acid sequence identity in their extracellular regions.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 312830
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD229/SLAMF3
Lys48-Phe454
Accession # AAH95921
Lys48-Phe454
Accession # AAH95921
Specificity
Detects mouse CD229/SLAMF3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CD229 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD229/SLAMF3 Antibody
Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD229/SLAMF3 Monoclonal Antibody (Catalog # MAB2555, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).Applications for Mouse CD229/SLAMF3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Western Blot
1 µg/mL
Sample: Recombinant Mouse CD229/SLAMF3 under non-reducing conditions only
Sample: Recombinant Mouse CD229/SLAMF3 under non-reducing conditions only
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD229/SLAMF3
Long Name
SLAM Family Member 3
Alternate Names
CD229, Ly9, SLAMF3
Gene Symbol
LY9
UniProt
Additional CD229/SLAMF3 Products
Product Documents for Mouse CD229/SLAMF3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CD229/SLAMF3 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
