Detects mouse CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) gamma c, recombinant human CD25/IL-2 R alpha, rmIL-2 R beta, or rmIL-15 R is observed.
Monoclonal Rat IgG2A Clone # 280406
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha Glu22-Lys236 Accession # P01590
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of CD25/IL-2 R alpha in CD4+ Mouse T cells by Flow Cytometry.
CD4+ mouse T cells treated with anti-mouse CD3 and anti-mouse CD28 for 24 hours were stained with Rat Anti-Mouse CD25/IL-2 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB2438A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2 R alpha
IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for Activation Induced Cell Death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12‑14).
Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
Miller, J. et al. (1985) J. Immunol. 134:4212.
Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
Hatakeyama, M. et al. (1989) Science 244:551.
Takeshita, T. et al. (1992) Science 257:379.
Wang, X. et al. (2005) Science 310:1159.
Wagner, D.K. et al. (1986) J. Immunol. 137:592.
Schulz, O. et al. (1998) J. Exp. Med. 187:271.
Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
Willerford, D.M. et al. (1995) Immunity 3:521.
Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Have you used Mouse CD25/IL-2 R alpha APC-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.