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Mouse CD25/IL-2 R alpha APC‑conjugated Antibody

  • Specificity
    Detects mouse CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) gamma c, recombinant human CD25/IL‑2 R alpha, rmIL-2 R beta, or rmIL-15 R is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 280406
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
    Glu22-Lys236
    Accession # P01590
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD25/IL-2 R alpha in CD4+ Mouse T cells by Flow Cytometry. CD4+ mouse T cells treated with anti-mouse CD3 and anti-mouse CD28 for 24 hours were stained with Rat Anti-Mouse CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB2438A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2 R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for Activation Induced Cell Death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12‑14).

  • References:
    1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
    2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
    3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
    4. Miller, J. et al. (1985) J. Immunol. 134:4212.
    5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
    6. Hatakeyama, M. et al. (1989) Science 244:551.
    7. Takeshita, T. et al. (1992) Science 257:379.
    8. Wang, X. et al. (2005) Science 310:1159.
    9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
    10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
    11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
    12. Willerford, D.M. et al. (1995) Immunity 3:521.
    13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
    14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    25704 (Human); 3559 (Human); 16184 (Human); 403870 (Canine);
  • Alternate Names:
    CD25; IL-2 R alpha; IL2RA; CD25; CD25 antigen; IDDM10; IL-2 receptor subunit alpha; IL2R; IL-2R subunit alpha; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy.
    Authors: Llopiz D, Dotor J, Casares N, Bezunartea J, Diaz-Valdes N, Ruiz M, Aranda F, Berraondo P, Prieto J, Lasarte JJ, Borras-Cuesta F, Sarobe P
    Int. J. Cancer, 2009;125(11):2614-23.
    Species: Mouse
    Application: Flow Cytometry,

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