Mouse CD27 Ligand/TNFSF7 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD27 Ligand/TNFSF7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human CD27 Ligand/TNFSF7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CD27 Ligand/TNFSF7
    Accession # O55237
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD27 Ligand/TNFSF7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) untreated or (B) treated with Recombinant Mouse CD40/TNFRSF5 Fc Chimera (Catalog # 1215-CD) and LPS were stained with Goat Anti-Mouse CD27 Ligand/TNFSF7 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB783P) and Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD27 Ligand/TNFSF7

CD27 Ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF Superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Mouse CD27L cDNA encodes a 195 amino acid (aa) residue protein with a 23 aa N-terminal cytoplasmic domain, a 21 transmembrane domain and a 151 aa C‑terminal extracellular domain. Mouse and human CD27L share approximated 56% aa sequence identity. By analogy to other TNFSF ligands, CD27L is expected to exist as non-covalent homotrimers. The expression of CD27L is highly regulated at the transcription and posttranslational level (3). CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. Although CD27L expression is not detected on human Dendritic Cells (DC), membrane expression on mature mouse DCs has been reported. CD27L expression is also present in the thymus medulla in both human and mouse. CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on Natural Killer (NK) cells and subsets of T and B cells (2, 4, 5). Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion, and the promotion of effector T cell formation (2). Ligation of CD27 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses (5, 6). On NK cells, ligation of CD27 induces proliferation and IFN-gamma production (4).

  • References:
    1. Hitzen, R.Q. et al. (1994) Immunol. Today 15:307.
    2. Croft, M. (2003) Nature Reviews Immunol. 3:609.
    3. Tesselaar, K. et al. (2003) J. Immunol. 169:33.
    4. Takeda, K. et al. (2000) J. Immunol. 164:1741.
    5. Kobata, T.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11249.
    6. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
  • Entrez Gene IDs:
    970 (Human); 21948 (Mouse)
  • Alternate Names:
    CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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