Detection of CD27 Ligand/TNFSF7 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes either (A) untreated or (B) treated with Recombinant Mouse CD40/TNFRSF5 Fc Chimera (Catalog # 1215-CD) and LPS were stained with Goat Anti-Mouse CD27 Ligand/TNFSF7 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB783P) and Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD27 Ligand/TNFSF7
CD27 Ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF Superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Mouse CD27L cDNA encodes a 195 amino acid (aa) residue protein with a 23 aa N-terminal cytoplasmic domain, a 21 transmembrane domain and a 151 aa C‑terminal extracellular domain. Mouse and human CD27L share approximated 56% aa sequence identity. By analogy to other TNFSF ligands, CD27L is expected to exist as non-covalent homotrimers. The expression of CD27L is highly regulated at the transcription and posttranslational level (3). CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. Although CD27L expression is not detected on human Dendritic Cells (DC), membrane expression on mature mouse DCs has been reported. CD27L expression is also present in the thymus medulla in both human and mouse. CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on Natural Killer (NK) cells and subsets of T and B cells (2, 4, 5). Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion, and the promotion of effector T cell formation (2). Ligation of CD27 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses (5, 6). On NK cells, ligation of CD27 induces proliferation and IFN-gamma production (4).
Hitzen, R.Q. et al. (1994) Immunol. Today 15:307.
Croft, M. (2003) Nature Reviews Immunol. 3:609.
Tesselaar, K. et al. (2003) J. Immunol. 169:33.
Takeda, K. et al. (2000) J. Immunol. 164:1741.
Kobata, T.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11249.
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