Mouse CD27/TNFRSF7 Antibody

Catalog # Availability Size / Price Qty
Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry.
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Product Details
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Mouse CD27/TNFRSF7 Antibody Summary

Species Reactivity
Detects mouse CD27 in direct ELISAs and Western blots. In Western blots; does not cross-react with recombinant human (rh) CD27, recombinant mouse (rm) CD40, rmGITR, rhDR6, rhTRAIL R4, rmHVEM, rmCD30, rmRANK, rmFas, rmTRAIL R2, rhTRADD, rm4-1BB, rmLT beta  R, rmOPG, rmNGFR, rmDR3, rmBAFF R, rhTRAIL R3, rmTAJ, rmEDAR, rmOX-40, rmTWEAK R, rmTNF R1, rhRELT,  or rmTRAIL R1.
Monoclonal Rat IgG2A Clone # 137915
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7
Accession # P41272
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below

Mouse CD27/TNFRSF7 Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CD27/TNFRSF7 Biotinylated Antibody (Catalog # BAM5741)

Standard: Recombinant Mouse CD27/TNFRSF7 Fc Chimera Protein, CF (Catalog # 574-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD27/TNFRSF7 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were labeled with Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB5741) followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113) and Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Immunocytochemistry CD27/TNFRSF7 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD27/TNFRSF7 in Mouse Splenocytes. CD27/TNFRSF7 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB5741) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

CD27 is a lymphocyte-specific member of the tumor necrosis factor receptor superfamily (TNFRSF) and is designated TNFRSF7 (1, 2). Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region (3). Mouse and human CD27 share approximately 65% amino acid identity. CD27 exists as homodimers on the cell surface via an extracellular disulfide bond in the membrane-proximal region. A soluble form of CD27 is also produced during the immune response and is found in various body fluids (4). CD27 is expressed on subsets of T and B cells. The expression of CD27 is upregulated upon T cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells (5). CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (1, 2). Ligation of CD27 on B cells has been shown to inhibit terminal differentiation of activated mouse B cells into plasma cells and enhances commitment to memory B cell responses (5).

  1. Croft, M. (2003) Nature Reviews Immunol. 3:609.
  2. Croft, M. (2003) Cytokine and Growth Factor Reviews 14:265.
  3. Gravestein, L.A. et al. (1993) Eur. J. Immunol. 23:943.
  4. Lens, S.M. et al. (1998) Semin. Immunol. 10:491.
  5. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
Entrez Gene IDs
939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
Alternate Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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