Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD302/CLEC13A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
Asp21-His165
Accession # Q9DCG2

Specificity

Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human CD302 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CD302/CLEC13A Antibody

Detection of Mouse CD302/CLEC13A antibody by Western Blot.

Detection of Mouse CD302/CLEC13A by Western Blot.

Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CD302/CLEC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6424) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD302/CLEC13A at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse CD302/CLEC13A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse spleen tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD302/CLEC13A

CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion and migration. Mature mouse CD302 is a 208 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 21-165) plus a 42 aa cytoplasmic region. The ECD possesses one C-type lectin domain (aa 30-149) that apparently cannot bind carbohydrate. In human, CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to this fusion protein. Mouse CD302 has two potential splice variants. One shows an alternative start site at Met61, while another shows a deletion of aa 154-162. Over aa 21-165, mouse CD302 shares 82% and 92% aa identity with human and rat CD302, respectively.

Alternate Names

BIMLEC, CD302, CLEC13A, DCL1

Entrez Gene IDs

9936 (Human); 66205 (Mouse); 295629 (Rat)

Gene Symbol

CD302

UniProt

Q9DCG2

Additional CD302/CLEC13A Products

Product Documents for Mouse CD302/CLEC13A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD302/CLEC13A Antibody

For research use only

Related Research Areas

C-type Lectin Family

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