Mouse CD39/ENTPD1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD39 and less than 1% cross-reactivity with rhCD39L2, rhCD39L3 and recombinant mouse CD39L3.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1
    Thr38-Ile478
    Accession # Q921Q6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse CD39/ENTPD1 (Catalog # 4398-EN), see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse CD39/ENTPD1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4398, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Immunohistochemistry
CD39/ENTPD1 in Mouse Liver. CD39/ENTPD1 was detected in perfusion fixed frozen sections of mouse liver using Mouse CD39/ENTPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4398) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (1), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (2). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (3). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (4). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (5).

  • References:
    1. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
    2. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
    3. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
    4. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
    5. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
  • Long Name:
    Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
  • Entrez Gene IDs:
    953 (Human); 12495 (Mouse); 64519 (Rat)
  • Alternate Names:
    ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

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Antigen Retrieval Reagent Sampler (50 mL each)

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Mounting Medium

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DAB Enhancer

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF016 7  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG Biotinylated Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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