Detection of CD45.1 in Mouse Splenocytes by Flow Cytometry.
SJL mouse splenocytes were stained with Mouse Anti-Mouse CD45.1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB8374N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (RIII, SJL/J, STS/A, DA).
Anderson, J.N. et al. (2004) FASEB J. 18:8.
Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
Cluster of Differentiation 45.1
Entrez Gene IDs:
5788 (Human); 19264 (Mouse)
B220; CD45 antigen; CD45.1; CD45R; EC 22.214.171.124; L-CA; LY5; Ly-5.1; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
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