Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD55/DAF Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF
Asp35-Pro359
Accession # Q61475

Specificity

Detects mouse CD55/DAF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CD55 and recombinant mouse CD97 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CD55/DAF Antibody

Detection of Mouse CD55/DAF antibody by Western Blot.

Detection of Mouse CD55/DAF by Western Blot.

Western blot shows lysates of mouse spleen tissue and mouse splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of CD55/DAF antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Detection of Mouse CD55/DAF by Western Blot

Detection of Mouse CD55/DAF by Western Blot

Glimepiride releases CD14 from RAW 264 cells. (A) The amounts of CD14 in RAW 264 cells treated for one hour with control medium (□) or glimepiride (■) as shown. Values are means ± SD, from triplicate experiments performed 4 times, n = 12. (B) The amounts of CD14 in supernatants from RAW 264 cells treated for one hour with control medium (□) or glimepiride as shown (■). Values are means ± SD, from triplicate experiments performed 4 times, n = 12. (C) Immunoblots showing the amounts of CD14, PrPC, CD55 and caveolin in extracts from RAW 264 cells treated for 1 hour with control medium (i) or 5 μM glimepiride (ii). (D) The amounts of CD14 in cells (□) or supernatants (■) from microglial cells treated for 1 hour with control medium, 5 μM glimepiride or 5 μM glipizide. Values are mean units CD14 ± SD, from triplicate experiments performed 3 times, n = 9. *Cellular CD14 significantly less than control cells. **supernatant CD14 significantly greater than control supernatants. (E) Blot showing the amounts of CD14 in supernatants from microglial cells treated with concentrations of glimepiride as shown for one hour. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24952384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD55/DAF Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

1 µg/mL
Sample: Mouse spleen tissue and Mouse splenocytes

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD55/DAF

CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules. It is found on blood cells, epithelium and endothelium and serves both as a receptor for CD97 and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-60 kDa, 356 amino acid (aa), GPI-linked form that is ubiquitously expressed. This molecule contains four SUSHI domains (aa 35-285), a Ser/Thr-rich region (aa 288-362) and a GPI-anchor at Gly362. There is also a 50 kDa, 379 aa, type I transmembrane form that is testis-associated. It shows the same domain architecture and is 93% aa identical to the GPI-form. At least four GPI gene isoforms exist. They diverge after Ile285 and show deletions and substitutions. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.

Alternate Names

CD55, CR, DAF, TC

Entrez Gene IDs

1604 (Human); 13136 (Mouse)

Gene Symbol

CD55

UniProt

Q61475 (Mouse)

Additional CD55/DAF Products

Product Documents for Mouse CD55/DAF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD55/DAF Antibody

For research use only

Related Research Areas

Complement Pathway

Citations for Mouse CD55/DAF Antibody

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