Mouse CD55/DAF Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse CD55/DAF by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Mouse CD55/DAF Antibody Summary

Species Reactivity
Detects mouse CD55/DAF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CD55 and recombinant mouse CD97 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF
Accession # Q61475
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CD55/DAF antibody by Western Blot. View Larger

Detection of Mouse CD55/DAF by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD55/DAF at approximately 75 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD55/DAF antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD55/DAF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5376, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD55/DAF

CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules. It is found on blood cells, epithelium and endothelium and serves both as a receptor for CD97 and a negative regulator of the C3 convertases, C4b2a and C3bBb. Mature mouse CD55 is the product of two genes that arose by duplication. There is a 55-60 kDa, 356 amino acid (aa), GPI-linked form that is ubiquitously expressed. This molecule contains four SUSHI domains (aa 35-285), a Ser/Thr-rich region (aa 288-362) and a GPI-anchor at Gly362. There is also a 50 kDa, 379 aa, type I transmembrane form that is testis-associated. It shows the same domain architecture and is 93% aa identical to the GPI-form. At least four GPI gene isoforms exist. They diverge after Ile285 and show deletions and substitutions. Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.

Entrez Gene IDs
1604 (Human); 13136 (Mouse)
Alternate Names
CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD55; CR; CRdecay accelerating factor for complement (CD55, Cromer blood group system); CROMDAFcomplement decay-accelerating factor; DAF; decay accelerating factor for complement; TC

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Citation for Mouse CD55/DAF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
    Authors: Ingham V, Williams A, Bate C
    J Neuroinflammation, 2014;11(0):115.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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