Mouse CD72 Antibody [Biotin]

R&D Systems | Catalog # BAF1279

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CD72 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD72
Arg117-Asn354
Accession # P21855

Specificity

Detects mouse CD72 in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human CD72 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD72 Antibody [Biotin]

CD72 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD72 in Mouse Splenocytes.

CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD72 Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD72

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD72

CD72, also named B-cell differentiation antigen Lyb-2, is a Type II transmembrane protein with a C-type lectin domain. It associates with CD5 and is a functional receptor for Semaphorin 4D in the immune system.

Alternate Names

CD72, Ly-19.2, Ly-32.2, Lyb-2

Entrez Gene IDs

971 (Human); 12517 (Mouse)

Gene Symbol

CD72

UniProt

P21855

Additional CD72 Products

Product Documents for Mouse CD72 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD72 Antibody [Biotin]

For research use only

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Protocols

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