Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD72 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD72
Arg117-Asn354
Accession # P21855

Specificity

Detects mouse CD72 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD72 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CD72 Antibody

CD72 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD72 in Mouse Splenocytes.

CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD72 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD72

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD72

CD72, also named B-cell differentiation antigen Lyb-2, is a Type II transmembrane protein with a C-type lectin domain. It associates with CD5 and is a functional receptor for Semaphorin 4D in the immune system.

Alternate Names

CD72, Ly-19.2, Ly-32.2, Lyb-2

Entrez Gene IDs

971 (Human); 12517 (Mouse)

Gene Symbol

CD72

UniProt

P21855

Additional CD72 Products

Product Documents for Mouse CD72 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD72 Antibody

For research use only

Citations for Mouse CD72 Antibody

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Protocols

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FAQs

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