Mouse CD83 PE-conjugated Antibody

FAB1437P has been discontinued. View all CD83 products.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD83 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD83
    Met22-Ala134
    Accession # O88324
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD83 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 100 ng/mL Recombinant Mouse GM‑CSF (Catalog # 415-ML) and 10 ng/mL Recombinant Mouse IL‑4 (Catalog # 404-ML) for 5 days, then with 100 ng/mL LPS overnight, were stained with Goat Anti-Mouse CD83 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1437P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD83

Mouse CD83 is a 30‑35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45‑55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

  • References:
    1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
    2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
    3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
    4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
    5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
    6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
    7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
    8. Kotzor, N. et al. (2004) Immunobiology 209:129.
    9. Zinser, E. et al. (2006) Immunobiology 21:449.
    10. Hirano, N. et al. (2006) Blood 107:1528.
    11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
    12. Yamashiro, S. et al. (2000) Blood 96:3958.
    13. Cao, W. et al. (2005) Biochem. J. 385:85.
    14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
    15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
  • Entrez Gene IDs:
    9308 (Human); 12522 (Mouse)
  • Alternate Names:
    B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Mouse CD83 PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Related Products
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee