Mouse Chk2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects endogenous mouse Chk2 in Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 235303
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Chk2
    Met1-Ala234
    Accession # Q9Z265
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Perfusion fixed frozen sections of mouse liver and lung
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Chk2 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Chk2 Monoclonal Antibody (Catalog # MAB22851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Chk2 at approximately 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk2

Checkpoint kinase 2 (Chk2) is a critical protein kinase involved in the cellular response to DNA damage. Chk2 is a target of the ATM protein kinase, and can phoshorylate multiple proteins, including Cdc25A and p53, to activate the G1/S cell cycle checkpoint. Patients with Li-Fraumeni syndrome, a cancer-prone familial disorder, may have a mutation in the chk2 or p53 genes, which suggests that Chk2 also functions as a tumor suppressor.

  • Long Name:
    Checkpoint Kinase 2
  • Entrez Gene IDs:
    11200 (Human); 50883 (Mouse); 114212 (Rat)
  • Alternate Names:
    CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog
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