|Detection of Mouse Chk2 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Chk2 Monoclonal Antibody (Catalog # MAB22851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Chk2 at approximately 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Checkpoint kinase 2 (Chk2) is a critical protein kinase involved in the cellular response to DNA damage. Chk2 is a target of the ATM protein kinase, and can phoshorylate multiple proteins, including Cdc25A and p53, to activate the G1/S cell cycle checkpoint. Patients with Li-Fraumeni syndrome, a cancer-prone familial disorder, may have a mutation in the chk2 or p53 genes, which suggests that Chk2 also functions as a tumor suppressor.
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